Luminescence detection of peptide:<i>N</i>-glycanase activity using engineered split inteins
Luminescence detection of peptide:<i>N</i>-glycanase activity using engineered split inteins
复制标题
使用工程化的分裂内含肽发光检测肽：<i>N</i>-聚糖酶活性
DOI：
10.1039/d2cc04865e
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发表时间：
2022
影响因子：
4.9
通讯作者：
Suzuki Tadashi
中科院分区：
文献类型：
--
作者：
Takahashi Tsuyoshi;Uchibayashi Tatsuya;Ishii Nozomi;Matsuo Ichiro;Yoshida Yukiko;Suzuki Tadashi
开发了一种基于断裂内含肽的方法来检测活细胞中的肽：N-聚糖酶（PNGase）活性。PNGase切割N，N′-二乙酰壳二糖与N-内含肽Asn侧链之间的连接，产物通过蛋白质反式剪接与C-内含肽快速反应，产生有活性的荧光素酶。
A split intein-based method has been developed to detect peptide:N-glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N′-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.