Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm
Several fibroblast growth factors are expressed during pre-attachment bovine conceptus development and regulate interferon-tau expression from trophectoderm
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DOI：
10.1530/rep-08-0396
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发表时间：
2009-02-01
期刊：
影响因子：
3.8
通讯作者：
Ealy, Alan D.
中科院分区：
文献类型：
--
作者：
Cooke, Flavia N. T.;Pennington, Kathleen A.;Ealy, Alan D.
滋养外胚层衍生因子干扰素tau（IFNT）维持牛和羊的子宫处于妊娠接受状态。成纤维细胞生长因子（FGF）参与调节IFNT的表达和其他与牛早期胚胎发育相关的潜在关键事件。这项工作的总体目标是确定各种FGF和FGF受体（FGFRs）在延长前附着牛孕体中表达，并确定这些FGF是否调节孕体发育和/或介导IFNT的产生。在妊娠第17天收集的体外衍生的牛囊胚和体内衍生的细长孕体表达至少四种FGF亚型（R1 c、R2 b、R3 c、R4）。此外，转录FGF 1，2和10，但没有FGF 7存在于延长牛的概念。FGF 10的表达模式与IFNT的表达模式最相似，两种转录物在第8天和第11天的妊娠中保持低水平，而在第14天和第17天的妊娠中显著增加。补充重组FGH，2或10增加牛滋养外胚层细胞和牛囊胚中IFNT mRNA水平，并增加滋养外胚层条件培养基中IFNT蛋白浓度。囊胚发育不受任何FGF的影响。总之，至少四种FGFR存在于附着前和附着周围的牛孕体中。此外，孕体表达至少三个候选FGF在延长，峰值IFN表达的时间。这些发现提供了新的见解如何概念衍生的因素，如FGH，2，和10可能控制IFNT表达在怀孕早期牛。
The trophectoderm-derived factor interferon tau (IFNT) maintains the uterus in a pregnancy-receptive state in cattle and sheep. Fibroblast growth factors (FGFs) are implicated in regulating IFNT expression and potentially other critical events associated with early conceptus development in cattle. The overall objectives of this work were to identify the various FGFs and FGF receptors (FGFRs) expressed in elongating pre-attachment bovine conceptuses and determine if these FGFs regulate conceptus development and/or mediate IFNT production. in vitro-derived bovine blastocysts and in vivo-derived elongated conceptuses collected at day 17 of pregnancy express at least four FGFRsubtypes (R1c, R2b, R3c, R4). In addition, transcripts for FGF1, 2, and 10 but not FGF7 are present in elongated bovine conceptuses. The expression pattern of FGF10 most closely resembled that of IFNT, with both transcripts remaining low in day 8 and day 11 conceptuses and increasing substantially in day 14 and day 17 conceptuses. Supplementation with recombinant FGH, 2 or 10 increased IFNT mRNA levels in bovine trophectoderm cells and bovine blastocysts and increased IFNT protein concentrations in trophectoderm-conditioned medium. Blastocyst development was not affected by any of the FGFs. In summary, at least four FGFRs reside in pre- and peri-attachment bovine conceptuses. Moreover, conceptuses express at least three candidate FGFs during elongation, the time of peak IFNTexpression. These findings provide new insight for how conceptus-derived factors such as FGH, 2, and 10 may control IFNTexpression during early pregnancy in cattle.