Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan
Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan
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DOI：
10.1111/j.1348-0421.2009.00152.x
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发表时间：
2009-09-01
影响因子：
2.6
通讯作者：
Katayama, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kitajima, Masaaki;Oka, Tomoichiro;Katayama, Kazuhiko
一个广泛的反应巢式RT-PCR检测MNV的发展，随后用于调查在日本的实验室小鼠MNV的流行。通过第二轮PCR在37份小鼠粪便标本中检测到8份（22%）MNV，尽管通过第一轮PCR从任何标本中均未获得阳性条带。第二轮PCR产物的遗传分析表明，本研究中检测到的MNV序列与MNV-3（DQ 223042）的序列密切匹配（97.2%对99.1%）。这是第一份证明MNV在日本流行的报告。
A broadly reactive nested RT-PCR assay to detect MNV was developed and subsequently used to investigate the prevalence of MNV in laboratory mice in Japan. MNV were detected in 8 (22%) of 37 murine stool specimens by second-round PCR, although no positive band was obtained from any specimen by first-round PCR. Genetic analysis of the second round PCR products showed that MNV sequences detected in this study were closely matched (97.2 similar to 99.1%) to that of MNV-3 (DQ223042). This is the first report demonstrating the prevalence of MNV in Japan.