Selective expression of adhesion molecules on human brain microvascular endothelial cells
Selective expression of adhesion molecules on human brain microvascular endothelial cells
复制标题
DOI：
10.1016/s0165-5728(97)00036-2
复制
发表时间：
1997-06-01
影响因子：
3.3
通讯作者：
Kim, KS
中科院分区：
文献类型：
--
作者：
Stins, MF;Gilles, F;Kim, KS
从儿童脑组织中分离出人微血管内皮细胞，观察其形态特征及细胞黏附分子对肿瘤坏死因子-α的反应。我们的人脑微血管内皮细胞(HBMEC)呈因子VIII-RAg、碳酸氢酶IV、Ulex Europe凝集素I阳性，摄取荧光标记的乙酰化低密度脂蛋白，表达γ-谷氨酰转肽酶，显示了其脑内皮细胞的特征。经肿瘤坏死因子α处理后，HBMEC仅表达少量的ELAM，而HUVEC表达明显的VCAM、ICAM和ELAM。这种细胞黏附分子的选择性表达也被脑组织的原位刺激所证实。结论：儿童脑微血管内皮细胞具有不同于大血管内皮细胞的细胞黏附分子的选择性表达。这可能会导致白细胞进入中枢神经系统。
Human microvascular endothelial cells were isolated from children's brain and examined for their morphological characteristics and upregulation of cell adhesion molecules in response to TNF alpha. Our human brain microvascular endothelial cells (HBMEC) were positive for factor VIII-Rag, carbonic anhydrase IV, Ulex Europeus Agglutinin I, took up fluorescently labeled acetylated low density lipoprotein and expressed gamma glutamyl transpeptidase, demonstrating their brain endothelial cell characteristics. Upon treatment with TNF alpha, VCAM and ICAM but little ELAM was expressed on HBMEC, while VCAM, ICAM and ELAM were clearly evident on HUVEC. This selective expression of cell adhesion molecules was also demonstrated by in situ stimulation of brain tissues. In conclusion, microvascular endothelial cells from childrens brains display selective expression of cell adhesion molecules, which differ from macrovascular endothelial cells. This may have consequences for leukocyte trafficking into the central nervous system.