Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis
Genotypes and haplotypes of CCR2 and CCR3 genes in Japanese cedar pollinosis
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DOI：
10.1159/000097502
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Eboshida, Akira
中科院分区：
文献类型：
--
作者：
Nakamura, Hiroyuki;Higashikawa, Fumiko;Eboshida, Akira
全基因组扫描分析表明，染色体3p21.3区域包含CC趋化因子受体的基因簇，可能在变态反应性炎症的发病机制中起关键作用。日本雪松花粉症是由一种I型变态反应介导的，在人类中引起季节性鼻炎和结膜炎，是日本春季最常见的花粉热形式，尽管雪松花粉症的候选基因仍未阐明。我们用聚合酶链式反应限制性片段长度多态性方法对雪松花粉症患者和对照组的CCR1、CCR2、CCR3、CCR5和CCXCR1进行了测序。CCXCR1的A111G、Arg127Cys和Arg252Gln，CCR1的T885C，CCR2的Val64Ile和T780C，CCR3的T51C和CCR5的Arg223Gln。对60例花粉症儿童及其父母的传递不平衡检验和对无亲缘关系的成人受试者(151名患者和157名对照)的关联研究表明，CCR2中的64Ile和CCR3中的51C与雪松花粉症显著相关。单倍型64Ile/780C/51C在花粉症患者中的频率显著高于对照组。我们的结果表明CCR2和CCR3基因是日本雪松花粉症的候选基因。版权所有(C)2007 S.Karger AG，巴塞尔。
Whole genome scan analyses have revealed that the chromosomal region 3p21.3, which contains a gene cluster of the CC chemokine receptor, is possibly critical for the pathogenesis of allergic inflammation. Japanese cedar pollinosis is mediated by a type I allergy and induces seasonal rhinitis and conjunctivitis in humans as the most common form of hay fever in spring in Japan, although the candidate genes for cedar pollinosis remain to be elucidated. We sequenced CCR1, CCR2, CCR3, CCR5, and CCXCR1 using the PCR restriction fragment length polymorphism method in subjects with cedar pollinosis and controls. We found 8 polymorphisms of A111G, Arg127Cys and Arg252Gln in CCXCR1, T885C in CCR1, Val64Ile and T780C in CCR2, T51C in CCR3 and Arg223Gln in CCR5. The transmission disequilibrium test using 60 children with pollinosis and their parents and an association study using unrelated adult subjects (151 patients and 157 controls) showed a significant association of 64Ile in CCR2 and 51C in CCR3 with cedar pollinosis. The frequency of haplotype 64Ile/780C/51C in pollinosis was significantly higher than in controls. Our results suggest that CCR2 and CCR3 genes are candidate genes for Japanese cedar pollinosis. Copyright (c) 2007 S. Karger AG, Basel.