G6PDH activity highlights the operation of the cyclic electron flow around PSI in Physcomitrella patens during salt stress.
G6PDH activity highlights the operation of the cyclic electron flow around PSI in Physcomitrella patens during salt stress.
复制标题
G6PDH 活性凸显了盐胁迫期间小立碗藓 PSI 周围循环电子流的运作
DOI：
10.1038/srep21245
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发表时间：
2016-02-18
影响因子：
4.6
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Gao S;Zheng Z;Huan L;Wang G
在盐胁迫和恢复过程中，立碗藓光合性能和葡萄糖-6-磷酸脱氢酶（G6 PDH）活性发生了较大变化。InP。光系统（PS）I的循环电子流比PS II更能耐受高盐胁迫。在高盐胁迫后，PSII活性恢复慢得多的PSI，这是迅速恢复到处理水平，即使PSII几乎被淹没。这一结果表明，盐胁迫后PSI周围的循环电子流的恢复是独立的PSII活性。此外，高盐胁迫下葡萄糖6磷酸脱氢酶活性和NADPH含量增加。当G6 PDH活性被G6 PDH抑制剂Glucm抑制时，PSI周围的循环电子流和NADPH含量显著降低。此外，在含有Glucm的液体培养基中恢复后，PSI活性远低于不含Glucm的液体培养基。这些结果表明PSI活性受G6 PDH活性和NADPH含量的显著影响。基于上述结果，我们提出G6 PDH在P.盐胁迫下，光合作用过程与光合作用过程密切相关，可能为盐胁迫下PSI周围的循环电子流提供NADPH，促进PSI的恢复。
Photosynthetic performances and glucose-6-phosphate dehydrogenase (G6PDH) activity inPhyscomitrella patenschanged greatly during salt stress and recovery. InP. patens, the cyclic electron flow around photosystem (PS) I was much more tolerant to high salt stress than PSII. After high salt stress, the PSII activity recovered much more slowly than that of PSI, which was rapidly restored to pretreatment levels even as PSII was almost inactivate. This result suggested that after salt stress the recovery of the cyclic electron flow around PSI was independent of PSII activity. In addition, G6PDH activity and NADPH content increased under high salt stress. When G6PDH activity was inhibited by glucosamine (Glucm, a G6PDH inhibitor), the cyclic electron flow around PSI and the NADPH content decreased significantly. Additionally, after recovery in liquid medium containing Glucm, the PSI activity was much lower than in liquid medium without Glucm. These results suggested the PSI activity was affected significantly by G6PDH activity and the NADPH content. Based on the above results, we propose that G6PDH inP. patenshas a close relationship with the photosynthetic process, possibly providing NADPH for the operation of the cyclic electron flow around PSI during salt stress and promoting the restoration of PSI.