Isolation and molecular characterization of thiosulfate-oxidizing bacteria from an Italian rice field soil
Isolation and molecular characterization of thiosulfate-oxidizing bacteria from an Italian rice field soil
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DOI：
10.1078/072320203322497482
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发表时间：
2003-09-01
影响因子：
3.4
通讯作者：
Stubner, S
中科院分区：
文献类型：
--
作者：
Graff, A;Stubner, S
在水稻土中，硫化合物发生活跃的循环。为了阐明硫代硫酸盐氧化细菌的多样性，采用液体培养基中的最大可能数法从土壤和水稻根部富集了这些细菌。从MPN富集培养物中，在固体矿物培养基上分离出21种细菌菌株，并且可以进一步显示从硫代硫酸盐产生硫酸盐。通过16 S rDNA分析对这些菌株进行了表征。这些菌株隶属于变形菌门α-和β-亚类的7个不同的系统发育类群，其中2个类群已被描述为该环境中的S-氧化菌（黄曲霉属和玻海属相关菌株），但5个类群代表稻田土壤中的新S-氧化菌。这些菌株与中生根瘤菌（Mesorhizobium loti）、噬氢菌（Hydrogenophaga sp.）到Delftia sp.，或显示与无色杆菌属的菌株的序列相似性。
In rice paddy soils an active cycling of sulfur compounds takes place. To elucidate the diversity of thiosulfate-oxidizing bacteria these organisms were enriched from bulk soil and rice roots by the most probable number method in liquid medium. From the MPN enrichment cultures 21 bacterial strains were isolated on solid mineral medium, and could be further shown to produce sulfate from thiosulfate. These strains were characterized by 16S rDNA analyses. The isolates were affiliated to seven different phylogenetic groups within the alpha- and beta-subclass of Proteobacteria.Two of these phylotypes were already described as S-oxidizers in this environment (Xanthobacter sp. and Bosea sp. related strains), but five groups represented new S-oxidizers in rice field soil. These isolates were closely related to Mesorhizobium loti, to Hydrogenophaga sp., to Delftia sp., to Pandoraea sp. or showed sequence similarity to a strain of Achromobacter sp.