Publisher Correction: Increasing ambient temperature progressively disassembles Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities.
Publisher Correction: Increasing ambient temperature progressively disassembles Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities.
复制标题
出版商更正：环境温度升高会逐渐将拟南芥光敏色素 B 从具有不同热稳定性的单个光体中分解出来。
DOI：
10.1038/s41467-020-16008-y
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发表时间：
2020
影响因子：
16.6
通讯作者：
Chen,Meng
中科院分区：
文献类型：
--
作者：
Hahm,Joseph;Kim,Keunhwa;Qiu,Yongjian;Chen,Meng
假设温暖的温度通过类似于遮荫的信号机制诱导植物热形态发生，因为两者都会破坏光感受器和热传感器光敏色素 B (phyB) 的活性形式。在细胞水平上，阴影通过触发 phyB 从光体分解来拮抗 phyB 信号传导。在这里，我们报告了拟南芥下胚轴和子叶表皮细胞中荧光蛋白标记的 phyB (phyB-FP) 的温度依赖性光体定位。我们的结果表明，温暖的温度会引发与阴影不同的光体动力学。温度从 12°C 升高到 27°C 通过刺激 phyB-FP 从选择性热不稳定光体中分解，逐渐减少光体数量。光体的热稳定性依赖于phyB的光传感模块。令人惊讶的是，尽管诱导了类似的光体动力学，但升高的温度对下胚轴和子叶中 phyB 的功能造成了相反的影响，表明组织/器官特异性温度信号传导电路位于光体动力学的下游或独立于 phyB。因此，我们的结果提供了直接的细胞生物学证据，支持通过具有不同热稳定性的单个光体的动态组装/分解来支持早期温度信号传导机制。
Warm temperature is postulated to induce plant thermomorphogenesis through a signaling mechanism similar to shade, as both destabilize the active form of the photoreceptor and thermosensor phytochrome B (phyB). At the cellular level, shade antagonizes phyB signaling by triggering phyB disassembly from photobodies. Here we report temperature-dependent photobody localization of fluorescent protein-tagged phyB (phyB-FP) in the epidermal cells ofArabidopsishypocotyl and cotyledon. Our results demonstrate that warm temperature elicits different photobody dynamics than those by shade. Increases in temperature from 12 °C to 27 °C incrementally reduce photobody number by stimulating phyB-FP disassembly from selective thermo-unstable photobodies. The thermostability of photobodies relies on phyB’s photosensory module. Surprisingly, elevated temperatures inflict opposite effects on phyB’s functions in the hypocotyl and cotyledon despite inducing similar photobody dynamics, indicative of tissue/organ-specific temperature signaling circuitry either downstream of photobody dynamics or independent of phyB. Our results thus provide direct cell biology evidence supporting an early temperature signaling mechanism via dynamic assembly/disassembly of individual photobodies possessing distinct thermostabilities.