Involvement of insulin-like growth factor-binding protein-related protein 1 in decidualization of human endometrial stromal cells
Involvement of insulin-like growth factor-binding protein-related protein 1 in decidualization of human endometrial stromal cells
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DOI：
10.1093/molehr/gam058
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发表时间：
2007-10-01
影响因子：
4
通讯作者：
Tamura, Kazuhiro
中科院分区：
文献类型：
--
作者：
Kutsukake, Masahiko;Ishihara, Ryosuke;Tamura, Kazuhiro
子宫蜕膜化对于成功着床和妊娠的建立至关重要。在本研究中，胰岛素样生长因子结合蛋白（IGFBP）相关蛋白1（IGFBP-rP 1）在人子宫和子宫内膜基质细胞（ESCs）的表达及其在蜕膜化的生理意义进行了研究。IGFBP-rP 1蛋白定位于子宫内膜的腺上皮、间质细胞和血管。培养的基质细胞表达IGFBP-rP 1并将其分泌到培养基中。IGFBP-rP 1主要定位于细胞质中，靠近细胞核。敲低内源性IGFBP-rP 1在基质细胞中的表达，通过一个小的干扰（si）RNA，减少了催乳素和IGFBP-1的表达，作为蜕膜标记。这些结果表明IGFBP-rP 1可能在胚胎干细胞的蜕膜化过程中发挥作用。
Uterine decidualization is crucial for successful implantation and the establishment of pregnancy. In the present study, the expression of insulin-like growth factor-binding protein (IGFBP)-related protein 1 (IGFBP-rP1) in the human uterus and endometrial stromal cells (ESCs) and its physiological significance in decidualization were examined. IGFBP-rP1 protein was localized in the glandular epithelium and stromal cells, and blood vessels in the endometrium. Cultured stromal cells expressed IGFBP-rP1 and secreted it into the medium. IGFBP-rP1 was localized mostly in the cytoplasm near the nucleus. Knocking down the endogenous IGFBP-rP1 expression in stromal cells, by a small interfering (si)RNA, diminished the expression of prolactin and IGFBP-1 which serve as decidual markers. These results suggest that IGFBP-rP1 may play a role in decidualization of ESCs.