DIMERIZATION OF SOLUBLE MAJOR HISTOCOMPATIBILITY COMPLEX PEPTIDE COMPLEXES IS SUFFICIENT FOR ACTIVATION OF T-CELL HYBRIDOMA AND INDUCTION OF UNRESPONSIVENESS
DIMERIZATION OF SOLUBLE MAJOR HISTOCOMPATIBILITY COMPLEX PEPTIDE COMPLEXES IS SUFFICIENT FOR ACTIVATION OF T-CELL HYBRIDOMA AND INDUCTION OF UNRESPONSIVENESS
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DOI：
10.1084/jem.182.2.439
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发表时间：
1995-08-01
影响因子：
15.3
通讯作者：
KOURILSKY, P
中科院分区：
文献类型：
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作者：
ABASTADO, JP;LONE, YC;KOURILSKY, P
主要组织相容性复合体（MHC）I类分子是向CD 8（+）T细胞呈递肽的细胞表面蛋白。这些肽主要来源于内源性合成的蛋白质。重组的可溶性MHC I类分子被生产、纯化并均匀地装载合成肽。这些MHC-肽复合物用于激活T细胞杂交瘤。虽然MHC-肽的单体与T细胞结合，但它们不显示刺激活性。二聚体完全触发T细胞杂交瘤分泌白细胞介素2。这种反应之后是一种状态，其中T细胞由于通过T细胞受体的信号转导缺陷而难以再刺激。
Major histocompatibility complex (MHC) class I molecules are cell-surface proteins that present peptides to CD8(+) T cells. These peptides are mostly derived from endogenously synthesized protein. Recombinant, soluble MHC class I molecules were produced, purified, and loaded homogeneously with synthetic peptide. These MHC-peptide complexes were used to activate a T cell hybridoma. While monomers of MHC-peptide bound to the T cell, they showed no stimulatory activity. Dimers fully triggered the T cell hybridoma to secrete interleukin 2. This response was followed by a state in which the T cell was refractory to restimulation as a result of defective signal transduction through the T cell receptor.