Optimization of Extraction Parameters of Total Phenolics from Annona crassiflora Mart. (Araticum) Fruits Using Response Surface Methodology
Optimization of Extraction Parameters of Total Phenolics from Annona crassiflora Mart. (Araticum) Fruits Using Response Surface Methodology
复制标题
DOI：
10.1007/s12161-016-0554-y
复制
发表时间：
2017-01-01
影响因子：
2.9
通讯作者：
Pastore, Glaucia Maria
中科院分区：
文献类型：
--
作者：
Arruda, Henrique Silvano;Pereira, Gustavo Araujo;Pastore, Glaucia Maria
在这项研究中，响应面法被用来优化提取温度（25-75摄氏度）和乙醇浓度（0- 70%，乙醇/水，v/v），以最大限度地提取总酚类化合物（TPC）从花生果肉。随着时间的推移监测提取过程的效率，在60-90 min之间达到平衡条件。二阶多项式模型与实验数据充分拟合，校正R（2）为0.9793（p < 0.0001），表明该模型可有效预测TPC含量。最佳提取条件为乙醇浓度46%（v/v）、提取温度75 A ° C、提取时间90 min。在最佳条件下，花生浆具有较高的TPC含量（4.67 g GAE/100 g dw），并且在所用的不同测定中也具有高抗氧化活性（DPPH IC 50、TEAC和T-ORAC（FL）分别为46.56 μ g/mL、683.65 μ mol TE/g和1593.72 μ mol TE/g）。从我们的提取程序中，我们成功回收了显著高于文献中其他研究的TPC量（高1.5-22倍）。此外，TPC和抗氧化活性存在于水果中的水平是很难找到其他常见的水果。这些结果揭示了一种潜在的方法，通过食用花生果改善人类健康。
In this study, response surface methodology was used to optimize the extraction temperature (25-75 A degrees C) and ethanol concentration (0-70 %, ethanol/water, v/v) to maximize the extraction of total phenolic compounds (TPC) from araticum pulp. The efficiency of the extraction process was monitored over time, and equilibrium conditions were reached between 60-90 min. A second-order polynomial model was adequately fit to the experimental data with an adjusted R (2) of 0.9793 (p < 0.0001) showing that the model could efficiently predict the TPC content. Optimum extraction conditions were ethanol concentration of 46 % (v/v), extraction temperature of 75 A degrees C and extraction time of 90 min. Under the optimum conditions, the araticum pulp showed high TPC content (4.67 g GAE/100 g dw) and also high antioxidant activity in the different assays used (46.56 mu g/mL, 683.65 mu mol TE/g and 1593.72 mu mol TE/g for DPPH IC50, TEAC and T-ORAC(FL), respectively). From our extraction procedure, we successfully recovered a significantly higher amount of TPC compared to other studies in the literature to date (1.5-22-fold higher). Furthermore, TPC and antioxidant activity were present in the fruit in levels that are difficult to find in other common fruits. These results expose a potential approach for improving human health through consumption of araticum fruit.