A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins
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DOI：
10.1038/nchem.1546
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发表时间：
2013-02-01
期刊：
影响因子：
21.8
通讯作者：
Johnsson, Kai
中科院分区：
文献类型：
--
作者：
Lukinavicius, Grazvydas;Umezawa, Keitaro;Johnsson, Kai
理想的生物成像荧光探针是明亮的，吸收长波长的，可以灵活地在活细胞和体内实施。然而，结合所有这些特性的合成荧光团的设计已被证明是极其困难的。在这里，我们介绍了一种生物相容性的近红外硅罗丹明探针，可以使用不同的标记技术特异性地偶联到蛋白质上。重要的是，其高渗透性和荧光特性允许对活细胞和组织中的蛋白质进行成像，其亮度和光稳定性使其非常适合于活细胞超分辨率显微镜。探针出色的光谱特性及其在活细胞应用中的易用性使其成为生物成像的强大新工具。
The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.