A NOVEL KINASE CASCADE TRIGGERED BY STRESS AND HEAT-SHOCK THAT STIMULATES MAPKAP KINASE-2 AND PHOSPHORYLATION OF THE SMALL HEAT-SHOCK PROTEINS
A NOVEL KINASE CASCADE TRIGGERED BY STRESS AND HEAT-SHOCK THAT STIMULATES MAPKAP KINASE-2 AND PHOSPHORYLATION OF THE SMALL HEAT-SHOCK PROTEINS
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DOI：
10.1016/0092-8674(94)90277-1
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发表时间：
1994-09-23
期刊：
影响因子：
64.5
通讯作者：
NEBREDA, AR
中科院分区：
文献类型：
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作者：
ROUSE, J;COHEN, P;NEBREDA, AR
MAPK活化蛋白激酶-2 （MAPKAP激酶-2）在体外被MAPK的p42和p44亚型（p42/p44(MAPK)）激活。然而，在一些细胞系中，MAPKAP激酶-2被亚砷酸钠、热休克或渗透胁迫激活，而不是被激活p42/p44（MAPK）的激动剂激活。我们已经确定了一种mapk样酶，它作为MAPKAP激酶-一种再激活激酶（RK）。RK被一种针对爪蟾MAPK （Mpk2）的抗血清识别，该蛋白与酿酒葡萄球菌的HOG1最相似。基于激活RK激酶或GST-Mpk2融合蛋白的能力，我们还鉴定了RK激酶（RKK）。RKK、RK和MAPKAP激酶-2在脊椎动物中构成了一条不同于经典的MAPK级联的应激激活信号转导新途径。
MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44(MAPK)). In several cell lines, however, MAPKAP kinase-2 is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that activate p42/p44(MAPK). We have identified a MAPK-like enzyme that acts as a MAPKAP kinase-a reactivating kinase (RK). RK is recognized by an antiserum raised against a Xenopus MAPK (Mpk2), which is most similar to HOG1 from S. cerevisiae. We also identified a RK kinase (RKK) on the basis of its ability to activate either RK or a GST-Mpk2 fusion protein. The RKK, RK, and MAPKAP kinase-2 constitute a new stress-activated signal transduction pathway in vertebrates that is distinct from the classical MAPK cascade.