Stimulation of Fusobacterium nucleatum biofilm formation by Porphyromonas gingivalis
Stimulation of Fusobacterium nucleatum biofilm formation by Porphyromonas gingivalis
复制标题
DOI：
10.1111/j.1399-302x.2007.00380.x
复制
发表时间：
2008-02-01
影响因子：
--
通讯作者：
Ishihara, K.
中科院分区：
文献类型：
--
作者：
Saito, Y.;Fujii, R.;Ishihara, K.
背景/目的：细菌感染是根尖周炎的主要原因。从根尖病变中清除这些微生物对于牙髓治疗的成功至关重要。本研究旨在阐明根尖周炎相关微生物与核梭杆菌、牙龈卟啉单胞菌之间的分子相互作用。方法：将从根尖周病变中分离出的微生物接种于I型胶原包裹的聚苯乙烯微滴定板中，在37℃厌氧条件下培养2天，然后用结晶紫染色检测平板上生物膜的数量。结果：核杆菌对I型胶原包被的聚苯乙烯微孔板具有很强的粘附性。牙龈假单胞菌能显著促进核假单胞菌生物膜的形成。它还得到了与牙龈假单胞菌隔室共培养的补充。牙龈假单胞菌对生物膜形成的促进作用仅因其自身诱导因子-2基因的失活而略有减弱。结论：牙龈假单胞菌通过释放自身诱导因子-2以外的可扩散的信号分子来促进其生物被膜的形成。
Background/aims: Bacterial infection is a major cause of periapical periodontitis. Eradication of these microorganisms from apical lesions is essential to the success of endodontic treatment. The aim of this study was to clarify the molecular interaction between Fusobacterium nucleatum, Porphyromonas gingivalis and other microorganisms associated with periapical periodontitis.Methods: Microorganisms isolated from periapical lesions were inoculated into type-I collagen-coated polystyrene microtiter plates and maintained at 37 degrees C under anaerobic conditions for 2 days, after which, the quantity of organized biofilm on the plates was evaluated by crystal violet staining. Growth enhancement via soluble factor was evaluated by separated coculture using a 0.4-mu m membrane filter.Results: F. nucleatum exhibited strong adherence to type-I collagen-coated polystyrene microplates. Biofilm formation by F. nucleatum was significantly enhanced by P. gingivalis. It was complemented by compartmentalized coculture with P. gingivalis. Enhancement of biofilm formation by P. gingivalis was only slightly reduced by inactivation of its autoinducer-2-producing gene luxS.Conclusion: The results suggest that P. gingivalis enhances biofilm formation by F. nucleatum by releasing diffusible signaling molecules other than autoinducer-2.