SURFACE-STRUCTURES (PERITRICHOUS FIBRILS AND TUFTS OF FIBRILS) FOUND ON STREPTOCOCCUS-SANGUIS STRAINS MAY BE RELATED TO THEIR ABILITY TO COAGGREGATE WITH OTHER ORAL GENERA
SURFACE-STRUCTURES (PERITRICHOUS FIBRILS AND TUFTS OF FIBRILS) FOUND ON STREPTOCOCCUS-SANGUIS STRAINS MAY BE RELATED TO THEIR ABILITY TO COAGGREGATE WITH OTHER ORAL GENERA
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DOI：
10.1128/iai.47.1.217-227.1985
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发表时间：
1985-01-01
影响因子：
3.1
通讯作者：
HESKETH, LM
中科院分区：
文献类型：
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作者：
HANDLEY, PS;CARTER, PL;HESKETH, LM
36株S.血链球菌生物型I和8株血链球菌。sanguis生物型II的表面结构的存在和它们与粘性放线菌、A. naeslundii和具核梭杆菌。负染色EM显示所有S上均可检测到表面结构。血细胞株大多数菌株（44个中的38个）携带周毛原纤维，其具有不规则的轮廓并且没有明显的宽度。它们通常表现为细胞周围具有恒定宽度的条纹。选择用于测量的菌株具有平均长度为72.4 ± 1.5的条纹。8.5生物型Ⅰ的平均粒径为51.6 ± 0.1nm。3.3 mm在生物型II上。一些纤维状生物型I菌株携带额外的、更长的（158.7 ± 0.0001）。33.1 nm）类型的原纤维突出通过较短的原纤维。原纤维密度是每种菌株的特征，范围从群体中所有细胞上的非常密集到群体中少数细胞上的非常稀疏。一小组6株进行了在细胞上的横向或极性位置的原纤维簇。取决于应变，在簇中存在1或2个长度的原纤维。一个菌株同时携带毛周原纤维和菌毛。菌毛是柔性结构，沿其整个沿着长度具有恒定的宽度（4.5-5.0 nm），但长度变化很大（≤ 1.0 nm）。0.7μ m）。S. sanguis I和II都包括具有周毛原纤维和成簇原纤维的菌株，但混合形态型菌株仅限于生物型II。对于测试的菌株，在整个生长周期的所有阶段，原纤维都存在于细胞上。新鲜分离的纤维状菌株与A. viscosus和A. naeslundii，尽管一些纤维状参考菌株缺乏这种能力。簇状型菌株不能共聚集，但混合型菌株共聚集良好。与F.具核质的纤维状菌株非常强，而簇状菌株的核质较弱。S. sanguis表面结构和共聚集能力进行了讨论。
Thirty-six strains of S. sanguis biotype I and 8 strains of S. sanguis biotype II were screened for the presence of surface structures and for their ability to coaggregate with Actinomyces viscosus, A. naeslundii and Fusobacterium nucleatum. Negative staining EM revealed detectable surface structures on all S. sanguis strains. The majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. They usually appeared as a fringe with a constant width around the cell. Strains selected for measurement had a fringe with an average length of 72.4 .+-. 8.5 nm on biotype I and 51.6 .+-. 3.3 mm on biotype II. Some fibrillar biotype I strains carried an additional, longer (158.7 .+-. 33.1 nm) type of fibril projecting through the shorter fibrils. Fibrillar density was characteristic for each strain, ranging from very dense on all cells in a population to very sparse on a few cells in a population. A small group of 6 strains carried tufts of fibrils in a lateral or polar position on the cell. Either 1 or 2 lengths of fibril were present in the tuft depending on the strain. One strain carried both peritrichous fibrils and fimbriae. Fimbriae are flexible structures with a constant width (4.5-5.0 nm) all along their length but very variable lengths (.ltoreq. 0.7 .mu.m) on each cell. S. sanguis I and II both included strains with peritrichous fibrils and tufts of fibrils, but the mixed morphotype strain was confined to biotype II. Fibrils were present on cells at all stages throughout the growth cycle for the strains tested. Freshly isolated fibrillar strains coaggregated consistently well wtih A. viscosus and A. naeslundii, although some fibrillar reference strains lacked the ability. No tufted strain could coaggregate, but the strains with the mixed morphotype coaggregated well. Coaggregation with F. nucleatum was very strong for the fibrillar strains, but less strong for the tufted strains. The possible correlation between S. sanguis surface structure and ability to coaggregate is discussed.