Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds.
Integrative analysis of metabolome and gut microbiota in diet-induced hyperlipidemic rats treated with berberine compounds.
复制标题
小檗碱化合物治疗饮食诱导的高脂血症大鼠代谢组和肠道微生物群的综合分析
DOI：
10.1186/s12967-016-0987-5
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发表时间：
2016-08-05
影响因子：
7.4
通讯作者：
Ji G
中科院分区：
文献类型：
--
作者：
Li M;Shu X;Xu H;Zhang C;Yang L;Zhang L;Ji G
背景高血压是代谢综合征的主要组成部分，常预示心血管疾病。我们开发了一种新的治疗剂小檗碱化合物（BC），由小檗碱，谷维素和维生素B6，并确定其抗高脂血症的活性和潜在的mechanism.MethodsMale Wistar大鼠喂高脂饮食（HFD）诱导高脂血症，然后给予BC口服4周。每周记录体重和食物摄入量，生化测定血清中的脂质谱。用GC-MS分析血清、尿液、肝脏和粪便中的代谢产物，并用16 S rDNA测序法测定其菌群结构。代谢组学分析表明，BC治疗导致血清中的维生素A酸、5-羟色胺、生酮和糖原氨基酸水平增加，尿液中的吡哆醇和4-吡哆酸增加，肝脏中的亚牛磺酸和蛋氨酸减少，粪便中的腐胺增加，脱氧胆酸盐和石胆酸盐减少。BC治疗也导致了有益细菌（如拟杆菌，布劳特氏菌）的富集和减少inEscherichias.ConclusionsThe降脂作用BC治疗高脂血症大鼠与脂质，碳水化合物和氨基酸的代谢，以及微生物群的结构的全球变化。
BackgroundHyperlipidemia is a major component of metabolic syndrome, and often predicts cardiovascular diseases. We developed a new therapeutic agent berberine compounds (BC), consisting of berberine, oryzanol and vitamin B6, and determined their anti-hyperlipidemia activity and underlying mechanisms.MethodsMale Wistar rats were fed a high fat diet (HFD) to induce hyperlipidemia, and then given BC orally for 4 weeks. Body weight and food intake were recorded weekly, and lipid profiles in serum were determined biochemically. Metabolites in serum, urine, liver and feces were analyzed by GC–MS, and the structure of microbiota was determined by 16S rDNA sequencing.ResultsLipid lowering was observed in the hyperlipidemic rats upon BC treatment without apparent adverse side effects. Metabolomics analysis indicated that the BC treatment resulted in increased pyruvic acid, serotonin, and ketogenic and glycogenic amino acid levels in the serum, increased pyridoxine and 4-pyridoxic acid in the urine, decreased hypotaurine and methionine in the liver, and increased putrescine and decreased deoxycholate and lithocholate in feces. The BC treatment also resulted in an enrichment of beneficial bacteria (e.g.Bacteroides,Blautia) and a decrease inEscherichia.ConclusionsThe lipid lowering effect of BC treatment in hyperlipidemic rats is associated with a global change in the metabolism of lipids, carbohydrates and amino acids, as well as the structure of microbiota.