THE T-PROTEIN ENCODED BY BRACHYURY IS A TISSUE-SPECIFIC TRANSCRIPTION FACTOR
THE T-PROTEIN ENCODED BY BRACHYURY IS A TISSUE-SPECIFIC TRANSCRIPTION FACTOR
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DOI：
10.1002/j.1460-2075.1995.tb00158.x
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发表时间：
1995-10-02
期刊：
影响因子：
11.4
通讯作者：
HERRMANN, BG
中科院分区：
文献类型：
--
作者：
KISPERT, A;KOSCHORZ, B;HERRMANN, BG
小鼠Brachyury（T）基因是脊索分化和中胚层形成所必需的。缺乏T活性的纯合子胚胎不发育躯干和尾巴，并在子宫内死亡。T基因在胚胎的脊索和早期中胚层细胞中特异性表达。最近的数据表明，T蛋白定位于细胞核中，并在体外特异性结合到20 bp的回文序列（T位点）。我们发现T蛋白通过与T位点结合激活HeLa细胞中报告基因的表达，因此T蛋白是一种新的组织特异性转录因子，它由一个大的N端DNA结合域组成（氨基酸1-229）和两对反式激活和阻遏结构域在C-末端蛋白半，T也可以通过各种取向和间隔的T位点反式激活转录，这一事实可能与寻找T蛋白控制的基因有关，并且在中胚层发育中很重要。
The mouse Brachyury (T) gene is required for differentiation of the notochord and formation of mesoderm during posterior development. Homozygous embryos lacking T activity do not develop a trunk and tail and die in utero. The T gene is specifically expressed in notochord and early mesoderm cells in the embryo. Recent data have demonstrated that the T protein is localized in the cell nucleus and specifically binds to a palindrome of 20 bp (the T site) in vitro. We show that the T protein activates expression of a reporter gene in HeLa cells through binding to the T site, Thus T is a novel tissue-specific transcription factor, It consists of a large N-terminal DNA binding domain (amino acids 1-229) and two pairs of transactivation and repression domains in the C-terminal protein half, T can also transactivate transcription through variously oriented and spaced T sites, a fact that may be relevant in the search for genes controlled by T protein and important in mesoderm development.