The generation of colony-forming cells (CFC) and the expansion of hematopoiesis in cultures of human cord blood cells is dependent on the presence of stem cell factor (SCF).
The generation of colony-forming cells (CFC) and the expansion of hematopoiesis in cultures of human cord blood cells is dependent on the presence of stem cell factor (SCF).
复制标题
人脐带血细胞培养物中集落形成细胞 (CFC) 的产生和造血功能的扩展取决于干细胞因子 (SCF) 的存在。
DOI：
10.1007/bf00748999
复制
发表时间：
1993
期刊：
影响因子：
2.2
通讯作者：
Adamson,JW
中科院分区：
文献类型：
--
作者：
Migliaccio,AR;Migliaccio,G;Durand,B;Mancini,GC;Adamson,JW
在无血清条件下，从光密度、大豆凝集素−、CD34+脐血细胞等方面分析了干细胞因子单独或与其他生长因子联合作用对体外培养的集落形成细胞和造血细胞扩增的影响。生长因子要么在培养开始时只添加一次，要么在培养物去菌后每隔几天添加一次，并用新鲜的培养基补充。在这些条件下，没有任何生长因子单独产生CFCs或扩大造血。然而，SCF与白细胞介素3(IL-3)或“迟反应因子”(粒细胞集落刺激因子(G-CSF)或促红细胞生成素(EPO))结合，可产生大量成熟细胞和CFCs。产生的氯氟化碳数量取决于采用的补给程序。在从未重新培养的培养物中，氯氟化碳的数量从第0天的>；160个氯氟化碳/培养物增加到第10天的>；3000个氯氟化碳。氯氟化碳数量在25天内保持在投入水平以上，然后下降。一个月后几乎没有检测到氯氟化碳。相比之下，在定期翻新的培养物中，至少40天都能检测到氯氟化碳。随着生长因子的不同，成熟细胞的谱系和生成的CFCs类型也不同。在SCF和IL-3的共同作用下，分化后的细胞有红系祖细胞和粒/巨噬细胞集落形成细胞，并存在红系和粒单核细胞前体细胞。相反，在SCF和G-CSF或EPO存在的情况下，祖细胞和分化的细胞受迟效生长因子(即SCF和G-CSF与BFU-E、红系集落形成细胞(CFU-E)和红系细胞在SCF和EPO存在时主要是G-CFCs和髓系细胞)的调控。因此，只需两个因子--SCF作为早期因子和适当的晚期作用因子--就可以在体外实现显著的红系和粒系扩增。
We have analyzed the effect of stem cell factor (SCF), alone or in combination with other growth factors, on the generation of colony-forming cells (CFC) and on the expansion of hematopoiesisin vitrofrom light density, soybean agglutinin−, CD34+cord blood cells under serum-deprived conditions. The growth factors were either added only once at the onset of the culture or added every few days when the cultures were demidepopulated and refed with fresh medium. No growth factor, alone, generated CFC or expanded hematopoiesis under these conditions. However, SCF, in combination with interleukin 3 (IL-3) or with “late-acting factors” (granulocyte colony-stimulating factor (G-CSF) or erythropoietin (Epo)), generated large numbers of mature cells as well as CFC. The number of CFC generated depended on the refeeding procedure adopted. In cultures never refed, the CFC numbers increased from > 160 CFC/culture at day 0 to > 3000 CFC at day 10. The CFC numbers stayed above the input levels for 25 days before declining. Almost no CFC were detectable after one month. In contrast, in cultures regularly refed, CFC were detectable for at least 40 days. The lineages of the mature cells and the types of CFC generated varied with the different growth factors. In the presence of SCF plus IL-3, erythroid burst-forming cells (BFU-E) and granulocyte/macrophage colony-forming cells (GM-CFC) were generated and erythroid as well as myelomonocytic precursors were present among the differentiated cells. In contrast, in the presence of SCF and G-CSF or Epo, the progenitor cells as well as the differentiated cells were dictated by the late-acting growth factor (i.e. mostly G-CFC and myeloid cells in the presence of SCF and G-CSF vs. BFU-E, erythroid colony-forming cells (CFU-E) and erythroblasts in the presence of SCF and Epo). Thus, marked expansion of erythropoiesis and granulopoiesis can be achievedin vitroby as few as two factors — SCF acting as the early factor along with the appropriate late-acting factor.