Maxillary Sinus Floor Elevation Using a Tissue-Engineered Bone with Calcium-Magnesium Phosphate Cement and Bone Marrow Stromal Cells in Rabbits
Maxillary Sinus Floor Elevation Using a Tissue-Engineered Bone with Calcium-Magnesium Phosphate Cement and Bone Marrow Stromal Cells in Rabbits
复制标题
使用含有钙镁磷酸盐水泥和骨髓基质细胞的组织工程骨进行上颌窦底抬高术
DOI：
10.1089/ten.tea.2011.0379
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发表时间：
2012-04-01
影响因子：
4.1
通讯作者：
Jiang, Xinquan
中科院分区：
文献类型：
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作者：
Zeng, Deliang;Xia, Lunguo;Jiang, Xinquan
本研究的目的是评估用骨髓基质细胞（bMSC）和磷酸钙镁水泥（CMPC）材料构建的组织工程骨提升上颌窦底的效果。采用电感耦合等离子体原子发射光谱法（ICP-AES）检测磷酸钙骨水泥（CPC）、磷酸镁骨水泥（MPC）和CMPC释放的钙（Ca）、镁（Mg）和磷（P）离子，并通过MTT分析、碱性磷酸酶（ALP）活性测定测定接种在CPC、MPC和CMPC上或在CPC、MPC和CMPC提取物中培养的bMSC的增殖和成骨分化，茜素红矿化测定，以及成骨基因 ALP 和骨钙素 (OCN) 的实时 PCR 分析。最后，将bMSC与CPC、MPC和CMPC联合用于兔上颌窦底抬高，而不含细胞的CPC、MPC或CMPC作为对照组。术后第2周和第8周通过组织学检查和荧光标记检测各组的新骨形成情况。观察到CMPC和CPC支架的Ca离子浓度显着高于MPC支架，而CMPC和MPC支架的Mg离子浓度显着高于CPC。接种在 CMPC 和 MPC 上或在其提取物中培养的 bMSC 分别比接种在 CPC 或在其提取物中培养的细胞增殖得更快。与接种在MPC或在其提取物中培养的bMSC相比，接种在CMPC和CPC上或在相应提取物中培养的bMSC的成骨分化显着增强；然而，CMPC 和 CPC 之间没有显着差异。对于体内上颌窦底抬高，CMPC较CPC和MPC能促进更多的新骨形成和矿化，而bMSC的添加可进一步显着增强其新骨形成能力。我们的数据表明，CMPC 具有中等的生物降解性和优异的骨传导性，这可能归因于其 Ca 和 Mg 离子成分，由 CMPC 和 bMSC 构建的组织工程骨可能是颌面骨再生的潜在替代移植物。
The objective of this study was to assess the effects of maxillary sinus floor elevation with a tissue-engineered bone constructed with bone marrow stromal cells (bMSCs) and calcium-magnesium phosphate cement (CMPC) material. The calcium (Ca), magnesium (Mg), and phosphorus (P) ions released from calcium phosphate cement (CPC), magnesium phosphate cement (MPC), and CMPC were detected by inductively coupled plasma atomic emission spectroscopy (ICP-AES), and the proliferation and osteogenic differentiation of bMSCs seeded on CPC, MPC, and CMPC or cultured in CPC, MPC, and CMPC extracts were measured by MTT analysis, alkaline phosphatase (ALP) activity assay, alizarin red mineralization assay, and real-time PCR analysis of the osteogenic genes ALP and osteocalcin (OCN). Finally, bMSCs were combined with CPC, MPC, and CMPC and used for maxillary sinus floor elevation in rabbits, while CPC, MPC, or CMPC without cells served as control groups. The new bone formation in each group was detected by histological finding and fluorochrome labeling at weeks 2 and 8 after surgical operation. It was observed that the Ca ion concentrations of the CMPC and CPC scaffolds was significantly higher than that of the MPC scaffold, while the Mg ions concentration of CMPC and MPC was significantly higher than that of CPC. The bMSCs seeded on CMPC and MPC or cultured in their extracts proliferated more quickly than the cells seeded on CPC or cultured in its extract, respectively. The osteogenic differentiation of bMSCs seeded on CMPC and CPC or cultured in the corresponding extracts was significantly enhanced compared to that of bMSCs seeded on MPC or cultured in its extract; however, there was no significant difference between CMPC and CPC. As for maxillary sinus floor elevation in vivo, CMPC could promote more new bone formation and mineralization compared to CPC and MPC, while the addition of bMSCs could further enhance its new bone formation ability significantly. Our data suggest that CMPC possesses moderate biodegradability and excellent osteoconductivity, which may be attributed to its Ca and Mg ion composition, and the tissue-engineered bone constructed of CMPC and bMSCs might be a potential alterative graft for maxillofacial bone regeneration.