Cryofocusing in the combination of gas chromatography with equilibrium headspace sampling
Cryofocusing in the combination of gas chromatography with equilibrium headspace sampling
复制标题
气相色谱与平衡顶空进样相结合的冷冻聚焦
DOI：
--
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
L. Ettre
中科院分区：
文献类型：
--
作者：
B. Kolb;B. Liebhardt;L. Ettre
摘要当利用毛细管柱的顶空气相色谱法进行痕量分析时，通常需要对样品进行富集。这涉及相当大的气体体积的不分流样品注射和相对较长的采样时间。因此，样品蒸气的谱带可能太大，导致峰失真和分辨率差。通过使用低温捕获可以轻松克服这个问题。虽然这可以通过在进样过程中将整个色谱柱冷却至低于环境温度来实现，但更方便的方法是利用毛细管柱第一个盘管的一部分作为低温捕集器。本文讨论了低温捕集的理论背景和仪器，并通过大量示例论证了其可能性。
SummaryWhen headspace gas chromatography utilizing capillary columns is used for trace analysis, sample enrichment is often needed. This involves splitless sample injection of fairly large gas volumes and relatively long sampling times. As a result of this, the band of the sample vapor may be too large causing peak distortion and poor resolution. This problem can be easily overcome by the use of cryogenic trapping. While this can be accomplished by cooling the whole column to subambient temperature during sample introduction, a more convenient way is to utilize part of the first coil of the capillary column as a cryogenic trap.The paper discusses the theoretical background and instrumentation of cryogenic trapping and demonstrates the possibilities through a number of examples.