Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid
Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid
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DOI：
10.1128/jcm.36.4.1117-1121.1998
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发表时间：
1998-04-01
影响因子：
9.4
通讯作者：
Bourhy, H
中科院分区：
文献类型：
--
作者：
Crepin, P;Audry, L;Bourhy, H
应用优化的逆转录-聚合酶链反应（RT-PCR）方法，对28例疑似狂犬病患者（其中9例经尸检证实为狂犬病）的临床标本进行了检测。使用唾液的RT-PCR结合皮肤活检样本进行的免疫荧光检测，允许在9名患者中检测狂犬病。
An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluorescence assay performed with skin biopsy samples allowed detection of rabies in the nine patients.