Effects of prenatal ethanol exposure on the development of Bergmann glia and astrocytes in the rat cerebellum: an immunohistochemical study.
Effects of prenatal ethanol exposure on the development of Bergmann glia and astrocytes in the rat cerebellum: an immunohistochemical study.
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产前乙醇暴露对大鼠小脑伯格曼胶质细胞和星形胶质细胞发育的影响：一项免疫组织化学研究。
DOI：
10.1002/cne.903210103
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Phillips,DE
中科院分区：
文献类型：
--
作者：
Shetty,AK;Phillips,DE
用胶质细胞酸性蛋白（GFAP）免疫标记技术研究了出生前乙醇暴露对大鼠小脑Bergmann胶质细胞和星形胶质细胞发育的影响。妊娠大鼠在整个妊娠期间喂食含乙醇的流质饮食（6.7%v/v）或配对喂食等热量饮食。在出生后第15天和第22天，对雌性后代小脑蚓部的旁脑切片进行GFAP免疫组织化学处理，以评估I、VII和X小叶中Bergmann胶质细胞和星形胶质细胞以及白色物质中央核心中星形胶质细胞的发育。在PD 15时，与对照动物相比，乙醇暴露动物每单位长度分子层的GFAP阳性Bergmann神经胶质纤维较少;形态学上不成熟的Bergmann纤维的百分比显著更大;每单位面积内部颗粒层和中央白色物质的GFAP阳性星形胶质细胞面积显著更大;星形胶质细胞突起更宽且更紧密堆积。这些胶质细胞变化与所有3个小叶中明显增厚的外部颗粒层相关。然而，在PD 22时，乙醇暴露组和对照组动物之间未观察到显著差异，表明乙醇暴露组动物在出生后第3周出现“追赶性生长”。这些结果表明，产前乙醇暴露导致（1）延迟成熟的Bergmann胶质细胞，这反过来又有助于延迟迁移的颗粒细胞;（2）在正常的出生后发育的星形胶质细胞的改变。© 1992 Wiley利斯公司
The consequences of prenatal ethanol exposure on the postnatal development of Bergmann glia and astrocytes in the rat cerebellum were investigated by using glial fibrillary acidic protein (GFAP) immunolabeling. Pregnant rats were either fed with an ethanol containing liquid diet (6.7% v/v) or pair‐fed with an isocaloric diet throughout gestation. On postnatal day (PD) 15 and 22, parasagittal sections of the cerebellar vermis from female offspring were processed for GFAP immunohistochemistry to assess the development of Bergmann glia and astrocytes in lobules I, VII, and X and astrocytes in the central core of white matter. On PD 15, compared to control animals, ethanol exposed animals had fewer GFAP positive Bergmann glial fibers per unit length of molecular layer; a significantly greater percentage of morphologically immature Bergmann fibers; a significantly greater GFAP positive astrocytic area per unit area of internal granular layer and central white matter; and the astrocytic processes were wider and more closely packed. These glial changes were associated with significantly thicker external granular layer in all 3 lobules. However, no significant differences were seen between the ethanol exposed and control animals on PD 22, indicating “catch‐up growth” in the ethanol exposed animals during the third postnatal week. These results suggest that prenatal ethanol exposure causes (1) delayed maturation of Bergmann glia, which in turn contributes to the delayed migration of granule cells; and (2) alterations in the normal postnatal development of astrocytes. © 1992 Wiley‐Liss, Inc.