Size-dependent electrophoretic migration and separation of liposomes by capillary zone electrophoresis in electrolyte solutions of various ionic strengths.
Size-dependent electrophoretic migration and separation of liposomes by capillary zone electrophoresis in electrolyte solutions of various ionic strengths.
复制标题
在各种离子强度的电解质溶液中通过毛细管区带电泳进行尺寸依赖性电泳迁移和脂质体分离。
DOI：
10.1021/ac000661e
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发表时间：
2000
影响因子：
7.4
通讯作者：
Chrambach,A
中科院分区：
文献类型：
--
作者：
Radko,SP;Stastna,M;Chrambach,A
研究了毛细管区带电泳（CZE）中脂质体的电泳迁移和分离特性。将脂质体挤出和非挤出制剂，其由各种比例的磷脂酰胆碱/磷脂酰甘油/胆固醇组成，平均直径范围为125至488 nm。当具有相同表面电荷密度的脂质体在不同离子强度（0.001 - 0.027）的Tris-HCl（pH 8）缓冲液中进行CZE时，它们按其大小顺序迁移。通过降低缓冲液的离子强度，可以增强或引起CZE中脂质体的尺寸依赖性电泳迁移和分离。结果表明，尺寸依赖性迁移主要是κR的函数，其中κ-1是双电层的厚度（可以从缓冲液的离子强度I导出），R是脂质体半径。脂质体迁移率取决于κ兰德表面电荷密度，其方式与Overbeek-Booth电动力学理论的预期一致。因此，松弛效应似乎是脂质体的尺寸依赖性电泳分离的物理机制。
The size-dependent electrophoretic migration and separation of liposomes was demonstrated and studied in capillary zone electrophoresis (CZE). The liposomes were extruded and nonextruded preparations consisting of phosphatidylcholine/phosphatidylglycerol/cholesterol in various ratios and ranging from 125 to 488 nm in mean diameter. When liposomes of identical surface charge density were subjected to CZE in Tris-HCl (pH 8) buffers of various ionic strengths (0.001−0.027), they migrated in order of their size. Size-dependent electrophoretic migration and separation of liposomes in CZE can be enhanced or brought about by decreasing the ionic strength of the buffer. It was shown that size-dependent migration is primarily a function of κR, where κ-1is the thickness of the electric double layer (which can be derived from the ionic strength,I, of the buffer) andR, the liposome radius. Liposome mobility depends on κRand surface charge density in a manner consistent with that expected from the Overbeek−Booth electrokinetic theory. Thus, the relaxation effect appears to be the physical mechanism underlying the size-dependent electrophoretic separation of liposomes.