MOLECULAR SUBTYPING OF PREVALENT M-SEROTYPES OF STREPTOCOCCUS-PYOGENES CAUSING INVASIVE DISEASE
MOLECULAR SUBTYPING OF PREVALENT M-SEROTYPES OF STREPTOCOCCUS-PYOGENES CAUSING INVASIVE DISEASE
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DOI：
10.1128/jcm.33.11.2850-2855.1995
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发表时间：
1995-11-01
影响因子：
9.4
通讯作者：
GEORGE, R
中科院分区：
文献类型：
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作者：
STANLEY, J;LINTON, D;GEORGE, R
针对英国的6种主要M血清型引起的侵袭性a链球菌病，定义了一种可重复的方法和高分辨率基因分型方案，将血清型参考菌株与来自肺炎、产褥期败血症、中毒性休克样综合征、蜂窝组织炎或坏死性筋膜炎等疾病患者的9种血清型临床分离株进行比较。评估了四种酶在16S rRNA基因特异性核糖分型中的区别性po，ver。EcoRI可产生血清型特异性核型，SacI可对同一M血清型的菌株进行细分，其鉴别能力最强。60株菌株中共发现25种组合核型，该方法的鉴别能力指数（D值）从M1血清型的0.51到M5血清型的0.98不等。采用少切内切酶smi和脉冲场凝胶电泳测定的大范围内切酶D值从M1血清型的0.37到M5血清型的1.0不等，大范围内切酶谱的比较显示M血清型间存在不同程度的遗传异质性，M1、M3、M6和M11菌株表现出克隆相关的大范围内切酶谱，而R28和M5菌株的大范围内切酶谱与多系起源一致。
Reproducible methodologies and a scheme for high-resolution genotyping of Streptococcus pyogenes were defined with respect to a study of six predominant M serotypes causing invasive group,A streptococcal disease in the United Kingdom, Serotype reference strains were compared with nine clinical isolates of each serotype from patients with diseases such as pneumonia, puerperal sepsis, toxic shock-like-syndrome, cellulitis, or necrotizing fasciitis, Four enzymes were evaluated for their discriminatory po,ver in 16S rRNA gene-specific ribotyping. Discriminatory power was greatest with EcoRI, which generated serotype-specific ribotypes, and with SacI, which could subdivide strains of the same M serotype, Twenty-five combined ribotypes were found among the 60 strains, and the indices of discriminatory power (D values) of this method varied from 0.51 within serotype M1 to 0.98 within strains of serotype M5, Macrorestriction with the rarely cutting endonuclease SmaI and pulsed-field gel electrophoresis gave D values varying from 0.37 within serotype M1 to the maximal 1.0 within serotype M5, Comparison of macrorestriction profiles revealed various degrees of genetic heterogeneity within M serotypes, Strains of M1, M3, M6, and M11 exhibited clonally related macrorestriction profiles, while those of R28 and M5 strains were consistent with polyphyletic origin.