Food intake in hypothalamic obesity.
Food intake in hypothalamic obesity.
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下丘脑肥胖的食物摄入量。
DOI：
10.1152/ajplegacy.1974.227.1.88
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
L. Frohman
中科院分区：
文献类型：
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作者：
J. Goldman;L. Bernardis;L. Frohman
材料和方法刚断奶的雄性Sprague-Dawley大鼠在到达时被分成两组，2天后，其中一组被置于每天4小时的喂养计划中（从8：00 PM到午夜），而另一组被允许连续获得食物。在该方案4天后，对所有动物称重并测量其体长。每组的一些大鼠进行下丘脑腹内侧核的电解破坏（病变），而在其余的电极插入VMN没有电流（假）。病变部位如前所述（8）。在病变放置后，所有动物均保持其各自的术前喂养计划。12天后，所有动物再次称重和测量。两天后处死动物前12小时取出笼内食物，取体重140 ~ 160 g的成年大鼠称重，并进行VMN破坏或假手术。本研究中所有假手术大鼠均在整个过程中自由进食，而具有破坏性损伤的大鼠被分为两组，其中一组自由进食，另一组与假损伤组每天成对进食。12天后，再次对大鼠称重并测量，2天后将其处死。自由采食大鼠在处死前可获得食物。
MATERIALS AND METHODSWeanling male Sprague-Dawley rats were divided into two groups on arrival and 2 days later one of these groups was placed on a 4-h daily feeding schedule (from 8: 00 PM to midnight), while the other group was allowed continuous access to food. After 4 days on this regimen all the animals were weighed and their body lengths were measured. A number of rats from each group was subjected to electrolytic destruction of the ventromedial hypothalamic nuclei (lesion), while in the remainder the electrode was inserted into the VMN without current flow (sham). The lesions were placed as previously described (8). Subsequent to the lesion placement, all the animals were maintained on their respective preoperative feeding schedules. Twelve days later all the animals were again weighed and measured. Two days later food was removed from the cages of all the animals 12 h before sacrifice.Mature rats weighing 140-l 60 g were weighed and measured and subjected to either VMN destruction or sham operations. All the sham-operated rats in this study were fed ad libitum throughout, whereas the rats with destructive lesions were divided into two groups, one of which was fed ad libitum and the other of which was pair fed on a daily basis with the sham-lesioned group. Twelve days later the rats were again weighed and measured and 2 days later they were sacrificed. The ad libitum-fed rats had food available until the time of sacrifice.