A radiosensitivity MiRNA signature validated by the TCGA database for head and neck squamous cell carcinomas.
A radiosensitivity MiRNA signature validated by the TCGA database for head and neck squamous cell carcinomas.
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DOI：
10.18632/oncotarget.5299
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发表时间：
2015-10-27
期刊：
影响因子：
--
通讯作者：
Xu B
中科院分区：
文献类型：
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作者：
Liu N;Boohaker RJ;Jiang C;Boohaker JR;Xu B
MicroRNA是一类小分子非编码RNA，在电离辐射（IR）诱导的DNA损伤的细胞应答中起着关键作用。越来越多的证据表明，响应于辐射，miRNA的改变控制DNA损伤反应途径中的细胞辐射敏感性。然而，关于miRNA调节放射敏感性的临床相关性尚不清楚。使用体外系统，我们进行了微阵列，以确定一个miRNA的签名，以评估放射敏感性。通过分析癌症基因组图谱（TCGA）数据库中可用的头颈部鳞状细胞癌（HNSCC）样本验证数据。共有27个miRNAs以共济失调-毛细血管扩张突变（ATM）激酶依赖性方式对IR反应显示差异性改变。我们验证了该列表，并确定了五个miRNA签名，可以预测HNSCC的辐射反应性。此外，我们发现ATM在这些患者中的表达水平与辐射反应性相关。总之，我们证明了使用miRNA签名来预测HNSCC放射治疗的临床反应性的可行性。
MicroRNA, a class of small non-coding RNAs, play critical roles in the cellular response to DNA damage induced by ionizing irradiation (IR). Growing evidence shows alteration of miRNAs, in response to radiation, controls cellular radiosensitivity in DNA damage response pathways. However, it is less clear about the clinical relevance of miRNA regulation in radiosensitivity. Using an in vitro system, we conducted microarray to identify a miRNA signature to assess radiosensitivity. The data were validated by analyzing available Head and Neck Squamous Cell Carcinoma (HNSCC) samples in the cancer genome atlas (TCGA) database. A total of 27 miRNAs showed differential alteration in response to IR in an Ataxia-Telangiectasia Mutated (ATM) kinase-dependent manner. We validated the list and identified a five miRNA signature that can predict radiation responsiveness in HNSCC. Furthermore, we found that the expression level of ATM in these patients was correlated with the radiation responsiveness. Together, we demonstrate the feasibility of using a miRNA signature to predict the clinical responsiveness of HNSCC radiotherapy.