Bioactive and Topographically-Modified Electrospun Membranes for the Creation of New Bone Regeneration Models
Bioactive and Topographically-Modified Electrospun Membranes for the Creation of New Bone Regeneration Models
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DOI：
10.3390/pr8111341
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发表时间：
2020-11-01
期刊：
影响因子：
3.5
通讯作者：
Asencio, Ilida Ortega
中科院分区：
文献类型：
--
作者：
Abdelmoneim, Dina;Alhamdani, Ghsaq M.;Asencio, Ilida Ortega
由创伤、癌症治疗或感染引起的骨损伤是一个重大且日益增长的全球性挑战。日益老龄化的人口在这方面起着关键作用，因为越来越多的骨折是由骨质疏松症等疾病引起的，这给医疗保健系统带来了负担。目前的修复策略没有充分考虑在健康组织中发现的细胞-底物相互作用；因此，对更复杂模型的需求是显而易见的。体外定义的3D微环境的创建是一种新兴的以地形为导向的方法，它为将细胞迁移和分化机制的知识应用于新细胞基质的创建提供了机会。此外，在体外骨再生模型中引入生物功能剂，在一定程度上可以控制细胞命运走向成骨途径。在这项研究中，我们应用了三种方法来功能化空间受限的静电纺丝人工微环境，这些微环境呈现了天然骨干细胞生态位的相关成分。研究了间充质基质细胞（MSCs）在细胞外基质（ECM）蛋白（胶原I）、糖胺聚糖（肝素）和陶瓷基材料（生物玻璃）功能化的电纺丝微支架上的生物学和成骨行为。在不改变聚己内酯（PCL）支架提供的纤维结构的情况下，成功地将胶原蛋白、肝素和生物玻璃（BG）纳入模型。我们成功地将间充质基质细胞（MSCs）植入到所有生物功能支架中，当暴露于PCL/BG复合材料时，它们的碱性磷酸酶产量增加。这项研究证明了制造智能和分层人工微环境用于研究干细胞行为的可行性，并最终将这些人工微环境纳入骨组织再生的多功能膜的潜力
Bone injuries that arise from trauma, cancer treatment, or infection are a major and growing global challenge. An increasingly ageing population plays a key role in this, since a growing number of fractures are due to diseases such as osteoporosis, which place a burden on healthcare systems. Current reparative strategies do not sufficiently consider cell-substrate interactions that are found in healthy tissues; therefore, the need for more complex models is clear. The creation of in vitro defined 3D microenvironments is an emerging topographically-orientated approach that provides opportunities to apply knowledge of cell migration and differentiation mechanisms to the creation of new cell substrates. Moreover, introducing biofunctional agents within in vitro models for bone regeneration has allowed, to a certain degree, the control of cell fate towards osteogenic pathways. In this research, we applied three methods for functionalizing spatially-confined electrospun artificial microenvironments that presented relevant components of the native bone stem cell niche. The biological and osteogenic behaviors of mesenchymal stromal cells (MSCs) were investigated on electrospun micro-fabricated scaffolds functionalized with extracellular matrix (ECM) proteins (collagen I), glycosaminoglycans (heparin), and ceramic-based materials (bioglass). Collagen, heparin, and bioglass (BG) were successfully included in the models without modifying the fibrous structures offered by the polycaprolactone (PCL) scaffolds. Mesenchymal stromal cells (MSCs) were successfully seeded in all the biofunctional scaffolds and they showed an increase in alkaline phosphatase production when exposed to PCL/BG composites. This research demonstrates the feasibility of manufacturing smart and hierarchical artificial microenvironments for studying stem cell behavior and ultimately the potential of incorporating these artificial microenvironments into multifunctional membranes for bone tissue regeneration