Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa model.
Lactobacillus crispatus inhibits growth of Gardnerella vaginalis and Neisseria gonorrhoeae on a porcine vaginal mucosa model.
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DOI：
10.1186/s12866-015-0608-0
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发表时间：
2015-12-09
期刊：
影响因子：
4.2
通讯作者：
Peterson ML
中科院分区：
文献类型：
--
作者：
Breshears LM;Edwards VL;Ravel J;Peterson ML
阴道微生物群会影响女性对细菌性阴道病 (BV) 和性传播感染 (STI) 的易感性。 BV 的特点是乳酸菌耗竭、厌氧菌过度生长（通常以阴道加德纳菌为主）和 pH>>4.5。 BV 与感染衣原体和淋病等性传播感染的风险增加有关。虽然这些关联已被确定，但导致感染风险的分子机制尚不清楚。开发了离体猪阴道粘膜模型（PVM）来探索乳杆菌属的机制作用。影响阴道加德纳菌和淋病奈瑟菌的定植。这里提供的数据表明，所有测试的生物体都可以在 PVM 上定殖并生长到临床相关的密度。此外，阴道加德纳菌和淋病奈瑟菌在 PVM 上形成生物膜。据观察，乳酸、乙酸和盐酸以 pH 依赖性方式抑制 PVM 上阴道加德纳菌的生长。淋病奈瑟菌在 pH 5.5 的乳酸存在下生长得最好，但在乙酸存在的情况下在此 pH 下生长不佳。最后，临床分离的卷曲乳杆菌 (24-9-7) 产生乳酸并抑制 PVM 上阴道加德纳菌和淋病奈瑟菌的生长。这些数据揭示了 pH 值、各种酸和卷曲乳杆菌对活阴道粘膜表面上的阴道加纳菌和淋病奈瑟菌生长的影响的差异。 PVM 是研究共生阴道微生物与病原体的相互作用以及阴道粘膜生物膜形成机制的有用模型。
The vaginal microbiota can impact the susceptibility of women to bacterial vaginosis (BV) and sexually transmitted infections (STIs). BV is characterized by depletion of Lactobacillus spp., an overgrowth of anaerobes (often dominated by Gardnerella vaginalis) and a pH > 4.5. BV is associated with an increased risk of acquiring STIs such as chlamydia and gonorrhea. While these associations have been identified, the molecular mechanism(s) driving the risk of infections are unknown. An ex vivo porcine vaginal mucosal model (PVM) was developed to explore the mechanistic role of Lactobacillus spp. in affecting colonization by G. vaginalis and Neisseria gonorrhoeae. The data presented here demonstrate that all organisms tested can colonize and grow on PVM to clinically relevant densities. Additionally, G. vaginalis and N. gonorrhoeae form biofilms on PVM. It was observed that lactic acid, acetic acid, and hydrochloric acid inhibit the growth of G. vaginalis on PVM in a pH-dependent manner. N. gonorrhoeae grows best in the presence of lactic acid at pH 5.5, but did not grow well at this pH in the presence of acetic acid. Finally, a clinical Lactobacillus crispatus isolate (24-9-7) produces lactic acid and inhibits growth of both G. vaginalis and N. gonorrhoeae on PVM. These data reveal differences in the effects of pH, various acids and L. crispatus on the growth of G. vaginalis and N. gonorrhoeae on a live vaginal mucosal surface. The PVM is a useful model for studying the interactions of commensal vaginal microbes with pathogens and the mechanisms of biofilm formation on the vaginal mucosa.