Metagenomic analysis of the turkey gut RNA virus community.
Metagenomic analysis of the turkey gut RNA virus community.
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DOI：
10.1186/1743-422x-7-313
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发表时间：
2010-11-12
期刊：
影响因子：
4.8
通讯作者：
Zsak L
中科院分区：
文献类型：
--
作者：
Day JM;Ballard LL;Duke MV;Scheffler BE;Zsak L
病毒性肠道疾病对全世界家禽生产者来说是一个持续的经济负担，尽管进行了大量的研究，但还没有任何一种病毒成为可能的病原体和预防和控制工作的目标。历史上，电子显微镜曾被用来识别可疑病毒，许多小而圆形的病毒无法仅根据形态进行分类。使用分子诊断进行的国家和地区调查显示，可疑病毒在美国家禽中持续传播，许多病毒同时出现在健康鸟类中。高通量核酸焦磷酸测序是一种强大的诊断技术，能够确定复杂环境样本中存在的完整基因组库。我们利用 Roche/454 Life Sciences GS-FLX 平台从患有肠道疾病的火鸡群中编译出 RNA 病毒宏基因组。这种方法在 BLAST nr 蛋白质数据库中产生了许多与病毒同源的序列，其中许多序列尚未在火鸡中得到描述。我们对火鸡肠道 RNA 宏基因组的分析特别关注小核糖核酸病毒目、杯状病毒科和火鸡小核糖核酸病毒的火鸡来源成员。
Viral enteric disease is an ongoing economic burden to poultry producers worldwide, and despite considerable research, no single virus has emerged as a likely causative agent and target for prevention and control efforts. Historically, electron microscopy has been used to identify suspect viruses, with many small, round viruses eluding classification based solely on morphology. National and regional surveys using molecular diagnostics have revealed that suspect viruses continuously circulate in United States poultry, with many viruses appearing concomitantly and in healthy birds. High-throughput nucleic acid pyrosequencing is a powerful diagnostic technology capable of determining the full genomic repertoire present in a complex environmental sample. We utilized the Roche/454 Life Sciences GS-FLX platform to compile an RNA virus metagenome from turkey flocks experiencing enteric disease. This approach yielded numerous sequences homologous to viruses in the BLAST nr protein database, many of which have not been described in turkeys. Our analysis of this turkey gut RNA metagenome focuses in particular on the turkey-origin members of the Picornavirales, the Caliciviridae, and the turkey Picobirnaviruses.