Antibiotic resistance and polymyxin B resistance mechanism of Aeromonas spp. isolated from yellow catfish, hybrid snakeheads and associated water from intensive fish farms in Southern China
Antibiotic resistance and polymyxin B resistance mechanism of Aeromonas spp. isolated from yellow catfish, hybrid snakeheads and associated water from intensive fish farms in Southern China
复制标题
气单胞菌的抗生素耐药性和多粘菌素 B 耐药机制。
DOI：
10.1016/j.lwt.2022.113802
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发表时间：
2022-08-02
影响因子：
6
通讯作者：
Meng, Hecheng
中科院分区：
文献类型：
--
作者：
Li, Lili;Yao, Ru;Meng, Hecheng
气单胞菌通常存在于水生环境中，它们可能导致海鱼患病，并与世界范围内与海产品有关的疫情有关。本研究从广东7个集约化水产养殖场中分离的200株气单胞菌（Aeromonas sp .）分别从表面健康的黄鲶鱼、杂交黑头鱼和相关水体中分离得到耐药菌株和多粘菌素B耐药机制。共鉴定出6种气单胞菌，其中以维罗氏单胞菌最为常见。采用肉汤微量稀释法对16种抗菌药物进行敏感性评价。所有分离株均对至少一种抗生素耐药，其中氨苄西林耐药率最高（99.0%），四环素（49.0%）和甲氧苄啶-磺胺甲恶唑耐药率相对较低（43.0%）。超过一半的菌株具有多重耐药（MDR）。7株菌株对多粘菌素B具有耐药性，最低抑菌浓度（MIC）为4 ~ 512 mg/L，由新型mcr-3样和mcr-7样基因介导。本研究结果表明，集约化养鱼场中的黄鲶鱼、杂交蛇头鱼及其相关环境是耐抗生素气单胞菌的宿主，可能污染相关鱼类产品并通过食物链传播给人类，构成潜在的公共卫生风险。
Aeromonas are often found in aquatic environments, in which they may cause disease in marine fish and are associated with seafood-related outbreaks worldwide. The objective of this study was to determine the antibiotic resistance and polymyxin B resistance mechanism of 200 isolates of Aeromonas spp. obtained from apparently healthy yellow catfish, hybrid snakehead and associated water, respectively, in seven intensive aquatic farms in Guangdong, China. Six Aeromonas species were identified, of which A. veronii was the most prevailing species. The susceptibility to 16 antimicrobial agents was evaluated by the broth microdilution method. All isolates were resistant to at least one antibiotic, with the highest resistance percentage to ampicillin (99.0%), and relative lower resistance rates to tetracycline (49.0%) and trimethoprim-sulfamethoxazole (43.0%). More than half of the strains were multi-drug resistant (MDR). Seven strains were resistant to polymyxin B with minimum inhibitory concentration (MIC) values of 4-512 mg/L, which were mediated by novel variable mcr-3-like and mcr-7-like genes. The results of this study indicated that yellow catfish, hybrid snakeheads and associated environments in intensive fish farms are reservoir of antibiotic resistant Aeromonas which may contaminate associated fish products and spread through food chain to human, representing a potential public health risk.