Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol
Lipid production in the under-characterized oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, from biodiesel-derived waste glycerol
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DOI：
10.1016/j.biortech.2015.02.051
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发表时间：
2015-06-01
影响因子：
11.4
通讯作者：
Cicek, Nazim
中科院分区：
文献类型：
--
作者：
Munch, Garret;Sestric, Ryan;Cicek, Nazim
在以甘油为碳源的限氮条件下，比较了两种产油酵母巴布耶瓦红冬孢酵母和二波瓦红冬孢酵母的生长、脂质产生和碳利用效率。生长 120 小时后，R. babjevae 和 R. diobovatum 的最终生物量浓度分别为 9.4 +/- 0.80 g/L 和 12.0 +/- 0.82 g/L。 R. babjevae 和 R. diobovatum 的最终脂质积累分别为 34.9 +/- 3.0% 细胞干重 (cdw) 和 63.7 +/- 4.5% cdw。在含有生物柴油衍生的废甘油的培养基中，R. babjevae 的生物量产量增加至 9.9 +/- 0.2 g/L，R. diobovatum 的生物量产量增加至 14.1 +/- 0.2 g/L，但两个物种的脂质积累均减少。在以生物柴油衍生的甘油作为碳源的 7 L 生物反应器中，R. diobovatum 产生了 13.6 +/- 0.4 g/L 生物量，并且积累的脂质达到 50.7 +/- 2.2% cdw，与烧瓶实验相匹配。 (C) 2015 Elsevier Ltd. 保留所有权利。
The growth, lipid production, and carbon use efficiency of two oleaginous yeasts, Rhodosporidium babjevae and Rhodosporidium diobovatum, were compared under nitrogen-limiting conditions with glycerol as the carbon source. Final biomass concentrations of R. babjevae and R. diobovatum were 9.4 +/- 0.80 g/L and 12.0 +/- 0.82 g/L, respectively, after 120 h of growth. Final lipid accumulation in for R. babjevae and R. diobovatum were 34.9 +/- 3.0% cell dry weight (cdw) and 63.7 +/- 4.5% cdw, respectively. Biomass production increased to 9.9 +/- 0.2 g/L for R. babjevae and 14.1 +/- 0.2 g/L for R. diobovatum in medium containing biodiesel-derived waste glycerol, but lipid accumulation decreased in both species. In a 7 L bioreactor with biodiesel-derived glycerol as carbon source, R. diobovatum produced 13.6 +/- 0.4 g/L biomass and accumulated lipids to 50.7 +/- 2.2% cdw, matching flask experiments. (C) 2015 Elsevier Ltd. All rights reserved.