Identification of differentially expressed genes by the cDNA-AFLP technique during dehydration of almond (Prunus amygdalus)
Identification of differentially expressed genes by the cDNA-AFLP technique during dehydration of almond (Prunus amygdalus)
复制标题
DOI：
10.1093/treephys/21.10.633
复制
发表时间：
2001-07-01
期刊：
影响因子：
4
通讯作者：
Messeguer, R
中科院分区：
文献类型：
--
作者：
Campalans, A;Pagès, M;Messeguer, R
我们分析了几个杏树(Prunus Amygdalus CL)脱水后强烈表达的基因。BATSCH)作为识别和确定参与抗旱机制的遗传基础的一种手段。利用杏仁作为研究木本植物脱水耐性的模式系统的优点包括其小的二倍体基因组和对干旱的适应。我们利用差异表达技术，即扩增限制性片段多态性衍生RNA指纹图谱技术，鉴定了成熟胚胎和经过干燥或脱落酸(ABA)处理的试管苗中积累的转录本。使用反向北极法来区分真差异条带和假阳性。一些预测的多肽与已发表的蛋白质序列相似，包括一个含氮化合物的转运体，一个1-酰基-sn-甘油-3-磷酸酰基转移酶，一个低分子量热休克蛋白，一个半胱氨酸蛋白酶和一个结构性表达的富含脯氨酸的蛋白。我们还比较了8个抗旱性不同的扁桃品种幼树叶片中已鉴定基因的表达水平。
We analyzed several genes that were strongly expressed in response to dehydration of almond (Prunus amygdalus CL.) Batsch) as a means of identifying and determining the genetic basis of mechanisms involved in drought tolerance. The advantages of using almond as a model system for studying dehydration tolerance in woody species include its small diploid genome and its adaptation to drought. We used the differential expression technique cDNA-AFLP (amplified restriction fragment polymorphism derived technique for RNA fingerprinting) to identify transcripts that accumulated in mature embryos and in in-vitro-cultured plantlets subjected to desiccation or abscisic acid (ABA) treatment. The reverse northern approach was used to differentiate between true differential bands and false positives. Some of the predicted polypeptides exhibited similarity to published protein sequences, including a transporter of nitrogenous compounds, a 1-acyl-sn-glycerol-3-phosphate acyl transferase, a low molecular weight heat shock protein, a cysteine proteinase and a constitutively expressed proline-rich protein. We also compared the levels of expression of the identified genes in leaves of young trees of eight almond cultivars differing in drought tolerance.