Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins
Enzymatic activity of toxic and non-toxic type 2 ribosome-inactivating proteins
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DOI：
10.1016/s0014-5793(04)00286-8
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发表时间：
2004-04-09
期刊：
影响因子：
3.5
通讯作者：
Stirpe, F
中科院分区：
文献类型：
--
作者：
Barbieri, L;Ciani, M;Stirpe, F
核糖体失活蛋白（RIPs）在不同的核酸底物上显示腺嘌呤多核苷酸糖基酶活性，这在核糖体水平上负责阻止蛋白质合成。一些2型rip，即蓖麻毒素和相关蛋白，对哺乳动物细胞和动物具有极大的毒性，而其他2型rip（无毒2型rip）的毒性则低三到四倍。我们研究了细胞毒性与核酸酶活性之间是否存在相关性。所有2型rip在不同底物上的去净活性不同，差异可达1至2 log。2型rip的毒性与它们在核酸或核糖体上的酶活性无关。(C) 2004年欧洲生化学会联合会。Elsevier B.V.版权所有。
Ribosome-inactivating proteins (RIPs) display adenine polynucleotide glycosylase activity on different nucleic acid substrates, which at the ribosomal level is responsible for the arrest of protein synthesis. Some type 2 RIPs, namely ricin and related proteins, are extremely toxic to mammalian cells and animals whilst other type 2 RIPs (non-toxic type 2 RIPs) display three to four logs less toxicity. We studied whether a correlation exists between toxicity on cells and enzymatic activity on nucleic acids. All type 2 RIPs differ in their depurinating activity on the different substrates with differences of up to one to two logs. The toxicity of type 2 RIPs is independent of their enzymatic activity on nucleic acids or on ribosomes. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.