Melatonin ameliorates acute necrotizing pancreatitis by the regulation of cytosolic Ca2+ homeostasis
Melatonin ameliorates acute necrotizing pancreatitis by the regulation of cytosolic Ca2+ homeostasis
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DOI：
10.1016/j.pan.2012.02.004
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发表时间：
2012-05-01
期刊：
影响因子：
3.6
通讯作者：
Huang, Zhiming
中科院分区：
文献类型：
--
作者：
Huai, Jiaping;Shao, Yingying;Huang, Zhiming
目的：本研究旨在探讨褪黑素对急性坏死性胰腺炎（ANP）大鼠胰腺的保护作用与肌浆网/内质网Ca ~（2+）-ATP酶（SERCA）和Na ~+/Ca ~（2+）交换器（NCX）表达的关系，以验证褪黑素是否通过减轻钙超载来改善ANP。将96只雄性SD大鼠随机分为假手术组、ANP组、褪黑素治疗组和褪黑素对照组。胆胰管逆行注射4%牛磺胆酸钠（1 ml/kg体重）诱发ANP。褪黑素治疗组在诱导ANP前30 min给予褪黑素（50 mg/kg体重）。在ANP诱导后1、4和8 h处死各组大鼠。结果：褪黑素治疗组各时间点血清SERCA、NCX水平均显著高于ANP组，低于假手术组和褪黑素对照组。这些水平在ANP组的4小时和8小时时间点之间没有差异。[Ca2结论：褪黑素可通过上调胰腺腺泡细胞内SERCA和NCX的表达，减轻胰腺腺泡细胞内钙超载，从而减轻胰腺损伤。版权所有（C）2012，IAP和EPC。由爱思唯尔印度出版，里德爱思唯尔印度私人有限公司的一个部门。保留所有权利。
Objectives: This study aims to investigate the relationship between the protective effects of melatonin in pancreas and the expression of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) and Na+/Ca2+ exchanger (NCX) in rats with acute necrotizing pancreatitis (ANP), to verify whether melatonin ameliorates ANP by alleviating calcium overload.Methods: Ninety-six male Sprague-Dawley rats were randomly divided into four groups (sham operation group, ANP group, melatonin treatment group, melatonin contrast group). ANP was induced by the retrograde injection of 4% taurocholate (1 ml/kg body weight) into the biliopancreatic duct. Melatonin (50 mg/kg body weight) was administered 30 min before the induction of ANP in the melatonin treatment group. Rats in each group were euthanized at 1, 4, and 8 h after ANP induction. Pancreatic tissues were removed to measure SERCA and NCX levels and cytosolic calcium ion (Ca2+) concentration ([Ca2+](i)).Results: At each time point, SERCA and NCX levels in the melatonin treatment group were significantly higher than that in the ANP group, and lower than that in the sham group and the melatonin contrast group. These levels did not differ between the 4- and 8-h time points in the ANP group. [Ca2+](i) in pancreatic acinar cells was higher in the melatonin treatment group than in the sham group and the melatonin contrast group, but lower than in the ANP group, at each time point.Conclusion: Melatonin can reduce pancreatic damage via the up-regulation of SERCA and NCX expression, which can alleviate calcium overload in pancreatic acinar cells. Copyright (C) 2012, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved.