Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.
Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.
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DOI：
10.1074/mcp.r120.002095
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Delafield DG;Li L
糖基化在生理事件和人类疾病中的日益重要性促使人们集中精力通过绝对和相对定量来揭示糖组学扰动。在开创性方法和不断提高的检测、鉴定和表征能力的推动下，过去十年中，聚糖和糖肽定量的合适策略数量显著增加。基于质谱的糖组定量策略已经发展到包括稳定同位素的代谢掺入、质量差和质量缺陷同位素标记的沉积以及同量异位素化学标记，为研究人员提供了准确和稳健定量的充足工具。除此之外，还设计了工作流程，以利用仪器的无标记定量能力，并开发了许多软件包，以促进可靠的光谱评分。在这篇综述中，我们提出并强调了化学标记和相关技术的聚糖和糖肽定量的最新进展。无标记、代谢、同位素和同量异序标记定量的新型糖组学应用研究定量糖组学的信息工具进入或扩展定量糖组学的关键考虑因素介绍合成简便、成本效益高的标记技术近年来，定量糖组学和糖蛋白组学的新策略出现了爆炸式增长。无论是通过稳定同位素的代谢掺入，定制同位素标记物的沉积，还是高通量同量异序化学标签，这些众多的新策略都为糖组学和糖蛋白质组学研究提供了便利。这篇综述强调了最近在标记方法，无标记策略，采集模式和生物信息学工具的聚糖和糖肽定量的创新，同时提供关键的评估和技术考虑，使有效的分析。
Growing implications of glycosylation in physiological occurrences and human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by seminal methodologies and increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. Mass-spectrometry-based strategies for glycomic quantitation have grown to include metabolic incorporation of stable isotopes, deposition of mass difference and mass defect isotopic labels, and isobaric chemical labeling, providing researchers with ample tools for accurate and robust quantitation. Beyond this, workflows have been designed to harness instrument capability for label-free quantification, and numerous software packages have been developed to facilitate reliable spectrum scoring. In this review, we present and highlight the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification. Novel glycomic applications of label-free, metabolic, isotopic, and isobaric labeling quantitation Informatic tools for investigative quantitative glycomics Critical considerations for entry or expansion of quantitative glycomics Introduction of synthetically facile, cost-effective labeling technology Recent years have seen an explosion in novel strategies for quantitative glycomics and glycoproteomics. Whether through metabolic incorporation of stable isotopes, deposition of custom isotopic labels, or high-throughput isobaric chemical tags, these numerous novel strategies provide ease of access to glycomic and glycoproteomic investigation. This review highlights the recent innovations in labeling methods, label-free strategies, acquisition modes, and bioinformatic tools for glycan and glycopeptide quantitation, while providing critical evaluations and technical considerations to enable effective analysis.
影响因子：
7.4
作者：
Kronewitter, Scott R.;Slysz, Gordon W.;Marginean, Ioan;Hagler, Clay D.;LaMarche, Brian L.;Zhao, Rui;Harris, Myanna Y.;Monroe, Matthew E.;Polyukh, Christina A.;Crowell, Kevin L.;Fillmore, Thomas L.;Carlson, Timothy S.;Camp, David G., II;Moore, Ronald J.;Payne, Samuel H.;Anderson, Gordon A.;Smith, Richard D.
通讯作者：
Smith, Richard D.