Angiotensin-converting enzyme (CD143) is abundantly expressed by dendritic cells and discriminates human monocyte-derived dendritic cells from acute myeloid leukemia-derived dendritic cells
Angiotensin-converting enzyme (CD143) is abundantly expressed by dendritic cells and discriminates human monocyte-derived dendritic cells from acute myeloid leukemia-derived dendritic cells
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DOI：
10.1016/j.exphem.2003.08.018
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发表时间：
2003-12-01
影响因子：
2.6
通讯作者：
Adema, GJ
中科院分区：
文献类型：
--
作者：
Danilov, SM;Sadovnikova, E;Adema, GJ
目标.血管紧张素转换酶（ACE）的表达模式在树突状细胞（DC）和巨噬细胞来源于正常单核细胞与DC来源于急性髓性白血病blasts.Materials和方法。通过流式细胞术使用一组针对ACE分子上的五种不同表位的单克隆抗体（mAb）并通过酶活性测量来定量ACE表达。一组抗ACE单克隆抗体与血细胞及其后代表面的结合模式，如通过FAGS所揭示的，显示谱系和表位特异性。单核细胞向巨噬细胞和DC的分化伴随着ACE表达的急剧增加。ACE活性在巨噬细胞中是单核细胞的50倍，在DC中是单核细胞的150倍。ACE水平归一化每个细胞显示，DC表达的ACE比单核细胞多1300倍。相反，从急性髓系白血病原始细胞衍生的DC没有显示出升高的ACE水平，尽管它们获得了DC标志物CD 80、CD
Objectives. The pattern of angiotensin-converting enzyme (ACE) expression in dendritic cells (DC) and macrophages derived from normal monocytes vs that in DC derived from acute myeloid leukemia blasts was investigated.Materials and Methods. ACE expression was quantified by flow cytometry using a set of monoclonal antibodies (mAbs) directed against five different epitopes on the ACE molecule and by enzyme activity measurement.Results. The binding pattern of a set of anti-ACE mAbs to the surface of blood cells and their progeny, as revealed by FAGS, showed lineage and epitope specificity. Differentiation of monocytes to macrophages and DC was accompanied by a dramatic increase in ACE expression. ACE activity was 50-fold higher in macrophages and 150-fold higher in DC than in monocytes. ACE level normalized per cell revealed that DC expressed 1300-fold more ACE than did monocytes. In contrast, DC derived from acute myeloid leukemia blasts did not show an elevated level of ACE, although they acquired DC markers CD80, CD