Both diet and gene mutation induced obesity affect oocyte quality in mice.
Both diet and gene mutation induced obesity affect oocyte quality in mice.
复制标题
饮食和基因突变引起的肥胖都会影响小鼠卵母细胞的质量。
DOI：
10.1038/srep18858
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发表时间：
2016-01-06
影响因子：
4.6
通讯作者：
Sun SC
中科院分区：
文献类型：
--
作者：
Hou YJ;Zhu CC;Duan X;Liu HL;Wang Q;Sun SC
肥胖被证明会导致生殖功能障碍，例如受孕减少、不孕和早孕流产。然而，肥胖对卵母细胞质量可能产生的影响仍未完全了解。在这项研究中，我们研究了饮食和基因突变引起的肥胖对小鼠卵母细胞极化、氧化应激和表观遗传修饰损伤的影响。我们的结果表明，高脂饮食诱导的肥胖（HFD）和基因突变诱导的肥胖（ob/ob）都会损害卵母细胞减数分裂成熟，破坏纺锤体形态，并降低卵母细胞极性。高 DHE 和 ROS 水平表明，肥胖小鼠的卵母细胞经历了氧化应激。在肥胖组小鼠的卵母细胞中观察到异常的线粒体分布和结构，并检测到早期凋亡信号，这表明氧化应激损害了线粒体功能并导致卵母细胞凋亡。我们的结果还表明，肥胖小鼠卵母细胞中 5 mC 水平以及 H3K9 和 H3K27 甲基化水平发生了改变，这表明 DNA 甲基化和组蛋白甲基化受到了影响。我们的结果表明，HFD 和 ob/ob 诱导的肥胖都会影响卵母细胞的成熟，氧化应激诱导的早期细胞凋亡和表观遗传修饰的改变可能是肥胖小鼠卵母细胞质量下降的原因。
Obesity was shown to cause reproductive dysfunctions such as reduced conception, infertility and early pregnancy loss. However, the possible effects of obesity on oocyte quality are still not fully understood. In this study we investigated the effects of both diet and gene mutation induced obesity on impairments in mouse oocyte polarization, oxidative stress, and epigenetic modifications. Our results showed that high-fat diet induced obesity (HFD) and gene mutation induced obesity (ob/ob) could both impair oocyte meiotic maturation, disrupt spindle morphology, and reduce oocyte polarity. Oocytes from obese mice underwent oxidative stress, as shown by high DHE and ROS levels. Abnormal mitochondrial distributions and structures were observed in oocytes from obese groups of mice and early apoptosis signals were detected, which suggesting that oxidative stress had impaired mitochondrial function and resulted in oocyte apoptosis. Our results also showed that 5 mC levels and H3K9 and H3K27 methylation levels were altered in oocytes from obese mice, which indicated that DNA methylation and histone methylation had been affected. Our results showed that both HFD and ob/ob induced obesity affected oocyte maturation and that oxidative stress-induced early apoptosis and altered epigenetic modifications may be the reasons for reduced oocyte quality in obese mice.