Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning:: Model for orally transmitted amyloidoses
Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning:: Model for orally transmitted amyloidoses
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DOI：
10.1111/j.1348-0421.2008.00054.x
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发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Onodera, Takashi
中科院分区：
文献类型：
--
作者：
Ano, Yasuhisa;Nakayama, Hiroyuki;Onodera, Takashi
为了研究病原菌在牛肠道内的掺入机制和朊蛋白的早期发病机制，在哺乳期和断奶期给牛口服a β - egfp融合蛋白。β - egfp通过乳牛回肠绒毛柱状上皮细胞掺入，并在隐窝斑块中积累。A - egfp的摄取和积累部位非常靠近周围神经系统；然而，在6个月断奶后的奶牛中没有观察到这种A - egfp的摄取。因此，目前的研究表明，断奶期对传播风险非常重要。
To determine the mechanism of bovine intestinal incorporation of the pathogen, and the pathogenesis of prion protein in the early stage, cows suckling and weaning were orally given a fusion protein of A beta-EGFP. A beta-EGFP was incorporated through the villous columnar epithelial cells and accumulated in crypt patches in the ileum of suckling cows. The sites of the uptake and accumulation of A beta-EGFP are very close to the peripheral nervous system; however, such uptake of A beta-EGFP was not observed in 6-month-old post-weaning cows. The present study, therefore, suggests that the weaning period is very important for the risk of transmission.