The RAB GTPase RABA1e localizes to the cell plate and shows distinct subcellular behavior from RABA2a under Endosidin 7 treatment
The RAB GTPase RABA1e localizes to the cell plate and shows distinct subcellular behavior from RABA2a under Endosidin 7 treatment
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DOI：
10.4161/15592324.2014.984520
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发表时间：
2016-01-01
影响因子：
2.9
通讯作者：
Drakakaki, Georgia
中科院分区：
文献类型：
--
作者：
Davis, Destiny J.;McDowell, Stephen C.;Drakakaki, Georgia
植物中的胞质分裂需要RAB GTP酶的活性来调节囊泡介导的物质对发育中的细胞板的贡献。虽然一些植物RAB GTP酶已被证明参与细胞板的形成，但许多仍在等待功能分配。在这里，我们报告了拟南芥中YFP-RABA 1 e的细胞板定位，并使用胞质分裂抑制剂Endosidin 7来提供与YFP-RABA 2a相比其定位的详细描述。在DMSO对照处理的晚期细胞板中观察到YFP-RABA 2a和YFP-RABA 1 e之间的差异，并且在内皮素7处理下变得更加明显。总之，我们的结果表明，个别RAB GTP酶可能作出不同的贡献，细胞板的形成，并进一步证明了实用的ES 7探针解剖它们。
Cytokinesis in plants requires the activity of RAB GTPases to regulate vesicle-mediated contribution of material to the developing cell plate. While some plant RAB GTPases have been shown to be involved in cell plate formation, many still await functional assignment. Here, we report cell plate localization for YFP-RABA1e in Arabidopsis thaliana and use the cytokinesis inhibitor Endosidin 7 to provide a detailed description of its localization compared to YFP-RABA2a. Differences between YFP-RABA2a and YFP-RABA1e were observed in late-stage cell plates under DMSO control treatment, and became more apparent under Endosidin 7 treatment. Taken together, our results suggest that individual RAB GTPases might make different contributions to cell plate formation and further demonstrates the utility of ES7 probe to dissect them.