Nanoscale dielectrophoretic spectroscopy of individual immobilized mammalian blood cells
Nanoscale dielectrophoretic spectroscopy of individual immobilized mammalian blood cells
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DOI：
10.1529/biophysj.106.082412
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发表时间：
2006-10-01
影响因子：
3.4
通讯作者：
Simpson, Garth J.
中科院分区：
文献类型：
--
作者：
Lynch, Brian P.;Hilton, Al M.;Simpson, Garth J.
对单个完整的表面固定的哺乳动物红细胞进行了介电泳力显微镜(DEPFM)和光谱分析。介电泳力谱在类似于125ms的时间内原位获得，并且可以在与扫描探针显微镜尖端的有效直径相当的区域内获得。介电泳谱的测量结果与单壳细胞模型的预测结果符合得很好。除了允许高度局部化的介电特性，DEPFM还提供了一种在水条件下对哺乳动物血细胞进行非接触成像的简单方法。这些研究证明了利用DEPFM以高空间分辨率实时监测固定化细胞膜电容的局部变化的可行性，补充了以前对移动全细胞和细胞悬液的研究。
Dielectrophoretic force microscopy (DEPFM) and spectroscopy have been performed on individual intact surface-immobilized mammalian red blood cells. Dielectrophoretic force spectra were obtained in situ in similar to 125 ms and could be acquired over a region comparable in dimension to the effective diameter of a scanning probe microscopy tip. Good agreement was observed between the measured dielectrophoretic spectra and predictions using a single-shell cell model. In addition to allowing for highly localized dielectric characterization, DEPFM provided a simple means for noncontact imaging of mammalian blood cells under aqueous conditions. These studies demonstrate the feasibility of using DEPFM to monitor localized changes in membrane capacitance in real time with high spatial resolution on immobilized cells, complementing previous studies of mobile whole cells and cell suspensions.