RECOMBINANT HUMAN INTERLEUKIN-5 IS A SELECTIVE EOSINOPHIL CHEMOATTRACTANT
RECOMBINANT HUMAN INTERLEUKIN-5 IS A SELECTIVE EOSINOPHIL CHEMOATTRACTANT
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DOI：
10.1002/eji.1830190420
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发表时间：
1989-04-01
影响因子：
5.4
通讯作者：
MANTOVANI, A
中科院分区：
文献类型：
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作者：
WANG, JM;RAMBALDI, A;MANTOVANI, A
人重组白细胞介素 (IL) 诱导人外周血嗜酸性粒细胞穿过聚碳酸酯过滤的迁移。使用带有聚碳酸酯和硝化纤维过滤器的棋盘设计来研究趋化性与趋化性的贡献。当不同的细胞因子浓度接种在过滤器上方和下方时，最大程度地诱导迁移需要室的下室和上室之间存在正浓度梯度，尽管发生了一些与梯度无关的迁移增强。这些结果表明诱导嗜酸性粒细胞穿过过滤器迁移涉及实际的趋化性。 IL 5 对嗜酸性粒细胞有选择性作用，对中性粒细胞和单核细胞没有作用。相反，粒细胞-巨噬细胞集落刺激因子引起嗜酸性粒细胞和中性粒细胞的迁移。因此，人IL 5是人嗜酸性粒细胞的有效且选择性的趋化剂。嗜酸性粒细胞在各种生理和病理条件下选择性地定位于组织中。局部产生的 IL 5 可能在从血液室选择性募集嗜酸性粒细胞中发挥作用。
Human recombinant interleukin (IL) induced migration across polycarbonate filterd of human peripheral blood eosinophils. The contribution of chemotaxis vs. chemokinesis was investigated using a checkerboard design with both polycarbonate and nitrocellulose filters. When different cytokine concentrations were seeded above and below the filter, maximal induction of migration required a positive concentration gradient between the lower and upper compartments of the chamber, though some gradient-independent augmentation of migration occurred. These results indicate that induction of eosinophil migration across filter involves actual chemotaxis. The effect of IL 5 was selective for eosinophils with no effect on neutrophils and monocytes. Conversely, granulocyte-macrophage colony-stimulating factor elicited migration of both eosinophils and neutrophils. Thus, human IL 5 is a potent and selective chemoattractant for human eosinophils. Eosinophils are selectively localized in tissues under a variety of physiological and pathological conditions. Locally produced IL 5 may play a role in the selective recruitment of eosinophils from the blood compartment.