ANALYSIS OF THE CHROMOSOMAL LOCALIZATION OF THE REPETITIVE SEQUENCES (RPSS) IN CANDIDA-ALBICANS
ANALYSIS OF THE CHROMOSOMAL LOCALIZATION OF THE REPETITIVE SEQUENCES (RPSS) IN CANDIDA-ALBICANS
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DOI：
10.1099/13500872-141-2-469
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发表时间：
1995-02-01
期刊：
影响因子：
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通讯作者：
TANAKA, K
中科院分区：
文献类型：
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作者：
CHINDAMPORN, A;NAKAGAWA, Y;TANAKA, K
在白色念珠菌菌株FC18的每条染色体上测定了白色念珠菌特异性RPS家族重复序列的位置和组织。利用脉冲场凝胶电泳，我们从8条染色体中分离出7个部分。用BamHI和XhoI切割每条染色体，去除RPS，然后用RPS探针杂交检测。除第4号染色体外，所有染色体均携带rps，这些rps位于每条染色体上的有限区域内。通过Sfil对每条染色体的酶切和RPS的探测，我们发现RPS区域内的这些识别位点在所有含有RPS的染色体中都是保守的。为了进一步表征rfs，我们在菌株FC18的6号染色体上检测了rfs的位置和边界区域。利用限制性内切酶Sfil、small、Xhol、BamHI、Mlul和Nrul，我们构建了该染色体上RPSs及其边界区域的半宏观物理图谱。我们还通过使用ROS的亚片段作为探针来确定RPS的哪个部分与每个边界相邻。给出了RPSs及其边界区域周围的物理结构，所得结果对进一步分析这些区域的功能有一定的参考价值。
The location and organization of repetitive sequences, members of the RPS family, which are sequences specific to Candida albicans, were determined on each chromosome of C. albicans strain FC18. Using pulsed-field gel electrophoresis, we separated seven fractions from eight chromosomes. Each chromosome was cleaved by BamHI and XhoI to excise the RPSs, which were then detected by hybridization with an RPS probe. All chromosomes except chromosome 4 carried RPSs, and these RPSs were located within a limited region on each chromosome. From the digestion of each chromosome with Sfil and probing with the RPSs, we found that these recognition sites within the RPS region were conserved among all RPS-containing chromosomes. For further characterization of the RPSs, the locations and the boundary regions of the RFSs were examined on chromosome 6 of strain FC18 as a model chromosome. Using the restriction enzymes Sfil, Smal, Xhol, BamHI, Mlul and Nrul, we constructed a semi-macro physical map of the RPSs and their boundary regions on this chromosome. We also determined which part of the RPS was adjacent to each boundary by using sub-fragments of ROS as probes. The physical configuration around the RPSs and their boundary regions are presented, The results obtained should be useful for future analysis of the function of these regions.