Effects of dietary synbiotic on growth, survival, lactobacillus bacterial count, blood indices and immunity of beluga (Huso huso Linnaeus, 1754) juvenile
Effects of dietary synbiotic on growth, survival, lactobacillus bacterial count, blood indices and immunity of beluga (Huso huso Linnaeus, 1754) juvenile
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DOI：
10.1111/anu.12219
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发表时间：
2015-12-01
影响因子：
3.5
通讯作者：
Jafarpour, S. A.
中科院分区：
文献类型：
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作者：
Akrami, R.;Nasri-Tajan, M.;Jafarpour, S. A.
本研究以白鲸幼鱼为试验对象，分别饲喂每千克饲料中添加1.0、2.0和4.0g的生物制剂，对照组不添加生物制剂，研究其对白鲸幼鱼生长发育的影响。饲养试验8周后，检测仔猪的生长性能、存活率、乳酸菌数、血液指标和免疫力。添加2.0g kg(-1)的合生素能显著提高仔鱼的生长性能和饲料利用率(WG、SGR和FCR)，但与对照组相比差异不显著(P>；0.05)。在肠道微生物区系方面，不同处理间的乳酸菌总数和乳酸菌数量差异不显著(P>；0.05)。此外，试验组之间的红细胞计数、红细胞压积、单核细胞、淋巴细胞、中性粒细胞、血糖和血清总蛋白水平无显著差异(P>；0.05)，而联合生元2组和4g·kg~(-1)组在白细胞计数和血红蛋白水平上有显著差异(P<；0.05)。此外，2.0g kg(-1)合生体饲料显著提高了仔鱼的补体活性(ACH50)和血清总免疫球蛋白(Ig)，但对溶菌酶活性无显著影响(P>；0.05)。这些结果表明，合生素在不影响生长性能的情况下提高了白鲟鱼的免疫力，调节了肠道微生物区系，并评估了基本的血液学参数。因此，建议添加2.0g kg(-1)的合生素作为白鲟鱼幼鱼的免疫促进剂。
This study aimed to determine the effect of synbiotic (Biomin IMBO) as feed additive on beluga (Huso huso) juvenile with an average body weight of 26.45 +/- 0.19 g. Experimental diets at which supplemented with 1.0, 2.0 and 4.0 g synbiotic per kg of feed were fed to beluga juvenile, whereas the control group was fed with no synbiotic. After the feeding trial for 8 weeks, growth performance, survival, lactobacillus bacterial count, blood indices and immunity were tested. Even though, some growth performance and feed utilization parameters including WG, SGR and FCR were improved in group fed with 2.0 g kg(-1) synbiotic; however, there were no significant differences (P > 0.05) in growth and feeding parameters between juveniles fed control and synbiotic supplementation diets. In terms of intestinal microbiota, there were no significant differences in total and lactic acid bacteria among treatments (P > 0.05). In addition, there were no significant differences of RBC counts, haematocrit, monocyte, lymphocyte, neutrophil, serum glucose and serum total protein levels between the treatment groups (P > 0.05), but group 2 and 4 g kg(-1) synbiotic showed a significant difference (P < 0.05) in WBC counts and haemoglobin, respectively. Furthermore, alternative complement activity (ACH50) and serum total immunoglobulin (Ig) were significantly increased in 2.0 g kg(-1) synbiotic fed fish (P < 0.05); however, it did not change the lysozyme activity, significantly (P > 0.05). These results indicate that synbiotic improves immunity of beluga sturgeon without detrimentally impacting the growth performance, modulates intestinal microbiota and basic haematological parameters assessed. Thus, we suggest that 2.0 g kg(-1) level of synbiotic may be used as an immunity promoter for beluga sturgeon juvenile.