Scavenger receptor MARCO contributes to macrophage phagocytosis and clearance of tumor cells
Scavenger receptor MARCO contributes to macrophage phagocytosis and clearance of tumor cells
复制标题
清道夫受体MARCO有助于巨噬细胞吞噬和清除肿瘤细胞
DOI：
10.1016/j.yexcr.2021.112862
复制
发表时间：
2021-10-11
影响因子：
3.7
通讯作者：
Zhou，Deshan
中科院分区：
文献类型：
--
作者：
Xing，Qianqian;Feng，Youxin;Zhou，Deshan
Macrophage receptor with collagenous structure (MARCO) is a member of the class A scavenger receptor family which is expressed on the cell surface of macrophages. It is well known that MARCO avidly binds to unopsonized pathogens, leading to its ingestion by macrophages. However, the role of MARCO in the recognition and phagocytosis of tumor cells by macrophages remains poorly understood. In this study, we used lentiviral technology to knockdown and overexpress MARCO and investigated the ability of macrophages to phagocytose tumor cells. Our results showed that MARCO expression was correlated with the ability of macrophages to carry on phagocytosis. MARCO knockdown led to significant decreases in the number of engulfment pseudopodia of macrophages and inhibition of the phagocytosis of tumor cells. On the other hand, MARCO overexpression elevated activity of SYK, PI3K and Rac1 in macrophages, which led to changes in macrophage morphology and enhanced phagocytosis by promoting formation of stress fibers and pseudopodia. By Co-IP analysis we showed that MARCO directly binds to the β5 integrin of SL4 tumor cells. In summary, these results demonstrated the important role for MARCO in demonstrated tumor cells uptake and clearance by macrophages.
