A New Polyester Technique for Sheet Plastination
A New Polyester Technique for Sheet Plastination
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DOI：
10.56507/jpvw6850
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发表时间：
2006-07
期刊：
影响因子：
--
通讯作者：
Hai-Bin Gao;Hong-Jin Sui
中科院分区：
文献类型：
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作者：
Hai-Bin Gao;Hong-Jin Sui
在本实验中，一具海角海豚尸体被用于评估一种新的聚酯薄板塑化技术。海豚被分为头部和身体两个区域。将组织在-70℃冷冻后，用高速带锯将头部切成43片3.0毫米厚的矢状切片。海豚的身体被切成348片相同厚度的横切片。所有切片均用10％甲醛固定，并用5％过氧化氢漂白。切片在冷丙酮浴中脱水，并在室温丙酮中脱脂。切片用霍芬聚酯（P45，中国）浸渍，并在两块玻璃板之间浇铸。然后将组织切片在热水浴中固化，而非用紫外线固化。制成的聚酯切片固化良好，并展示出详细的解剖信息。
A cape dolphin cadaver was used to evaluate a new polyester sheet plastination technique in this experiment. The dolphin was divided into two regions, the head and the body. After freezing the tissue at - 70℃, the head was cut into forty-three 3.0mm thick sagittal slices with a high-speed band saw. The body of the dolphin was cut into 348 transverse slices of the same thickness. All slices were fixed using 10％ formaldehyde and bleached using 5％ dioxogen. The slices were dehydrated in a cold acetone bath and degreased in room temperature acetone. The slices were impregnated with Hoffen Polyester (P45, China) and cast between two glass plates. Then tissue slices were cured in heated water bath instead of UV-light. The finished polyester slices were cured properly and exhibited detailed anatomical information.