Bile acid synthesis in man. II. Determination of 7 alpha-hydroxycholesterol, (22R)-22-hydroxycholesterol, and 26-hydroxycholesterol in human meconium.
Bile acid synthesis in man. II. Determination of 7 alpha-hydroxycholesterol, (22R)-22-hydroxycholesterol, and 26-hydroxycholesterol in human meconium.
复制标题
人体内胆汁酸的合成。
DOI：
10.1016/s0021-9258(19)69141-6
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发表时间：
1977
影响因子：
6.5
通讯作者：
N. Javitt
中科院分区：
文献类型：
--
作者：
U. Lavy;S. Burstein;M. Gut;N. Javitt
7 -羟胆固醇，(22R)-22-羟胆固醇和26-羟胆固醇已在人胎便定量。该方法采用四氢呋喃萃取和溶剂溶解硫酸盐酯，液相分配色谱法分离羟基甾醇，气相液相色谱法定量，气相液相色谱-质谱法鉴定，氚化和14c标记示踪剂作为整体回收标准。（22R）-22-羟胆固醇和26-羟胆固醇几乎全部（大于93%）存在于硫酸盐馏分中，浓度分别为3.8 ~ 6.4 mg / 100g胎粪和0.4 ~ 0.8 mg / 100g胎粪。由于游离氚化(22R)-22-羟基胆固醇被用作示踪剂来评估这种羟基甾醇的回收率，因此发现这种化合物的浓度可能很小。以氚化26-羟基胆固醇3,26-二磺酸盐为示踪剂测定该化合物的含量，并优化了26-羟基胆固醇的溶剂溶解回收率和7 α -羟基胆固醇的最低分解率。以不含示踪剂的羟甾醇为回收标准，未发现显著量的7 α -羟胆固醇。
7 alpha-Hydroxycholesterol, (22R)-22-hydroxycholesterol and 26-hydroxycholesterol have been quantitated in human meconium. The method used tetrahydrofuran for extraction and solvolysis of the sulfate esters, liquid partition chromatography for the separation of the hydroxysterols, gas-liquid chromatography for quantitation, gas-liquid chromatography-mass spectrometry for identification, and tritiated and 14C-labeled tracers for overall recovery standards. (22R)-22-Hydroxycholesterol and 26-hydroxycholesterol were present almost entirely, ( greater than 93%) in the sulfate fraction at concentrations of 3.8-6.4 and 0.4-0.8 mg per 100 g meconium, respectively. Since free tritiated (22R)-22-hydroxycholesterol was used as the tracer to assess recovery of this hydroxysterol, the concentrations found for this compound may be minimal. Tritiated 26-hydroxycholesterol 3,26-disulfate was used as tracer to determine the levels of this compound, and the solvolysis procedure was optimized for recovery of 26-hydroxycholesterol and least decomposition of 7 alpha-hydroxycholesterol. No significant amounts of 7 alpha-hydroxycholesterol were found based on the tracer-free hydroxysterol as recovery standard.