The ribosomal DNA of Calliphora erythrocephala; and analysis of hybrid plasmids containing ribosomal DNA.
The ribosomal DNA of Calliphora erythrocephala; and analysis of hybrid plasmids containing ribosomal DNA.
复制标题
红头丽丽的核糖体DNA；
DOI：
10.1016/0022-2836(80)90162-x
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发表时间：
1980
影响因子：
5.6
通讯作者：
R. White
中科院分区：
文献类型：
--
作者：
K. Beckingham;R. White
我们通过克隆到质粒载体中的一系列rDNA†片段分析了红头丽蝇（Calliphora erythrocephala）的核糖体DNA。我们绘制了这些质粒中8种限制性内切酶的位点图，并使用Berk和Sharp（1977）的S1内切酶作图程序在选定质粒的图谱中定位了18 S和28 S rRNA的编码区。这一分析表明，一些rDNA顺反子的C。红头绦虫含有一个“内含子”（吉尔伯特，1978），它在与D.黑胃DNA在这些克隆的片段中分离出两个长度为2.85个碱基的内含子和一个较长的序列相关变体的一部分。酶切位点分析和初步杂交结果表明，该菌株的2.85kb内含子为C. erythrocephalais在序列上与D.黑色素瘤DNA内含子。
We have analysed the ribosomal DNA ofCalliphora erythrocephala, a Dipteran fly of the same sub-order asDrosophila melanogaster, through a series of rDNA†fragments cloned in a plasmid vector. We have mapped the sites for eight restriction enzymes within these plasmids, and positioned the regions coding for the 18 S and 28 S rRNAs within the maps of selected plasmids using the S1endonuclease mapping procedure of Berk & Sharp (1977). This analysis establishes that some rDNA cistrons ofC. erythrocephalacontain an “intron” (Gilbert, 1978) which interrupts the 28 S coding region at the same position as that ofD. melanogasterrDNA. Two introns of 2.85 kilobases in length and part of a longer, sequence-related variant were isolated in these cloned fragments. Restriction enzyme site analysis and preliminary hybridization data indicate that the 2.85 kb intron ofC. erythrocephalais largely unrelated in sequence to the two classes ofD. melanogasterrDNA introns.