THE MORPHOGENESIS OF AVIAN TENDON
THE MORPHOGENESIS OF AVIAN TENDON
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DOI：
10.1098/rspb.1956.0011
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发表时间：
1956-01-01
期刊：
影响因子：
--
通讯作者：
JACKSON, SF
中科院分区：
文献类型：
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作者：
JACKSON, SF
通过电镜观察胚胎鸡跖腱的薄片显示，在8天的胚胎中，最早可确定的胶原原纤维直径为80 Å，与构成肌腱雏形的致密、明显合胞细胞的细胞质密切相关。随着发育的进行，一些细胞质内的原纤维群是可分辨的，但细胞间隙也发育并逐渐充满原纤维；最后，束形成并在相邻的细胞之间排列。很快，细胞外组织占主导地位，直到第20天，原纤维的平均直径为400 Å，胶原蛋白的正常周期为640 Å。所显示的形态特征与组织化学数据相关，并讨论了各种细胞成分在细胞间物质形成中的可能功能。通过使用原纤维与束轴完全横向切割的切片，可以看出每个原纤维都被纤维间物质包裹着。随着年龄的增长，原纤维直径增大，纤维束内纤维间物质的相对体积减小；因此得出结论，这种材料必须含有胶原蛋白或必要的前体，以解释原纤维的扩大。因此，纤维间物质对胶原原纤维的形成和生长至关重要。
Observations by electron microscopy on thin sections of the metatarsal tendon of embryonic fowls show that in the 8-day embryo the earliest definable collagen fibrils of 80 Å in diameter are intimately associated with the cytoplasm of the compact, apparently syncytial, cells of which the tendon rudiment is composed. As development proceeds, some intracytoplasmic groups of fibrils are distinguishable, but intercellular spaces also develop and these gradually become filled with fibrils; finally, bundles are formed and lie packed between the adjacent cells. Soon the extracellular organization predominates until at 20days the average diameter of the fibrils is 400 Å and the normal 640 Å periodicity of collagen has been achieved. The morphological features demonstrated have been correlated with histochemical data, and the possible function of the various cellular components in the formation of the intercellular substance has been discussed. By the use of sections in which fibrils have been cut exactly transverse to the bundle axis it has been shown that each fibril is invested by interfibrillar material. As the diameter of the fibrils increases with age the relative volume of interfibrillar material within a bundle diminishes; it is therefore concluded that this material must contain either collagen or the necessary precursors in order to account for the enlargement of the fibrils. Thus the interfibrillar material is of fundamental importance to the formation and growth of the collagen fibrils.