Catalytic properties of galactose oxidase to liposome-forming amphiphiles which have many pendent galactose residues
Catalytic properties of galactose oxidase to liposome-forming amphiphiles which have many pendent galactose residues
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半乳糖氧化酶对具有许多悬垂半乳糖残基的脂质体形成两亲物的催化特性
DOI：
10.1021/bc970166y
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发表时间：
1998
影响因子：
4.7
通讯作者：
Kitano
中科院分区：
文献类型：
--
作者：
Ohno;Kitano
以亲脂性自由基引发剂引发含半乳糖的乙烯基单体[2-（甲基丙烯酰氧基）乙基β-d-吡喃半乳糖苷（MEGal）]聚合。所得两亲物（DODA-PMEGal）与磷脂混合形成稳定的脂质体，脂质体表面的半乳糖残基被半乳糖氧化酶有效识别并氧化。半乳糖氧化酶对脂质体上半乳糖残基的亲和力（以1/Km值估算）高于对游离半乳糖和MEGal的亲和力，且与脂质体表面载半乳糖聚合物链的长度和膜的流动性有关，而不受脂质体上半乳糖残基的表面密度的影响。半乳糖氧化酶对由偶氮型自由基引发剂和链转移剂制备的含半乳糖线性聚合物的亲和力也高于对游离半乳糖和MEGal的亲和力，并依赖于MEGal的聚合度。
A galactose-carrying vinyl monomer [2-(methacryloyloxy)ethyl β-d-galactopyranoside, MEGal] was polymerized by using a lipophilic radical initiator. The amphiphiles obtained (DODA-PMEGal) formed stable liposomes by mixing with phospholipids, and the galactose residues on the liposome surface were effectively recognized and oxidized by galactose oxidase. The affinity (estimated by the 1/Km value) of galactose oxidase for the galactose residues on the liposomes was higher than those for free galactose and MEGal and dependent on the length of galactose-carrying polymer chains on the liposome surface and the fluidity of the membranes, whereas not significantly influenced by the surface density of galactose residues on the liposomes. The affinity of galactose oxidase for the galactose-carrying linear polymers, which were prepared by using an ordinary azo-type radical initiator and a chain-transfer reagent, was also higher than those for free galactose and MEGal and dependent on the degree of polymerization of M...