Solid-phase microextraction: a promising technique for sample preparation in environmental analysis.
Solid-phase microextraction: a promising technique for sample preparation in environmental analysis.
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DOI：
10.1016/s0021-9673(00)00453-2
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发表时间：
2000-08
期刊：
影响因子：
--
通讯作者：
M. F. Alpendurada
中科院分区：
文献类型：
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作者：
M. F. Alpendurada
固相微萃取(SPME)是一种简单有效的吸附和解吸技术，它不需要溶剂或复杂的仪器，就可以浓缩液体样品或顶空中的挥发性或非挥发性化合物。固相微萃取适用于通过气相色谱和高效液相色谱分离和检测分析物，并可为广泛浓度的分析物提供线性结果。通过控制纤维上涂层的极性和厚度，保持一致的采样时间，并调整其他提取参数，分析师可以确保低浓度分析物的高度一致、可量化的结果。到目前为止，关于SPME的文章已在不同领域发表了约400篇，包括环境(水、土壤、空气)、食品、天然产物、药品、生物学、毒理学、法医学和理论。随着固相微萃取范围的扩大，出现了新的涂层，从而提高了这种提取技术的专属性。SPME纤维的关键部分当然是纤维涂层。目前，有27种纤维涂层和浆料可供选择。其中最新的是具有悬浮在聚二甲基硅氧烷中的二乙烯基苯和Carboxen双重涂层的纤维组件，以及用于特定无隔膜注射系统的23号纤维系列。SPME的增长还反映在使该技术更易于使用的附件数量的不断增加中。此外，还提供了便携式现场取样器，这是一个独立的单元，可以在取样后和运送到实验室期间存储SPME纤维。一些科学出版物显示了在实验室间验证研究中获得的结果，在这些研究中，固相微萃取被用于确定ppt级别的不同有机化合物的存在，这证明了这种用于定量分析的提取技术的可靠性。
Solid-phase microextraction (SPME) is a simple and effective adsorption and desorption technique, which eliminates the need for solvents or complicated apparatus, for concentrating volatile or nonvolatile compounds in liquid samples or headspace. SPME is compatible with analyte separation and detection by gas chromatography and high-performance liquid chromatography, and provides linear results for wide concentrations of analytes. By controlling the polarity and thickness of the coating on the fibre, maintaining consistent sampling time, and adjusting other extraction parameters, an analyst can ensure highly consistent, quantifiable results for low concentration analytes. To date, about 400 articles on SPME have been published in different fields, including environment (water, soil, air), food, natural products, pharmaceuticals, biology, toxicology, forensics and theory. As the scope of SPME grew, new improvements were made with the appearance of new coatings that allowed an increase in the specificity of this extraction technique. The key part of the SPME fibre is of course the fibre coating. At the moment, 27 variations of fibre coating and size are available. Among the newest are a fibre assembly with a dual coating of divinylbenzene and Carboxen suspended in poly(dimethylsiloxane), and a series of 23 gauge fibres intended for specific septumless injection system. The growth of SPME is also reflected in the expanding number of the accessories that make the technology even easier to use Also available is a portable field sampler which is a self-contained unit that stores the SPME fibre after sampling and during the shipment to the laboratory. Several scientific publications show the results obtained in inter-laboratory validation studies in which SPME was applied to determine the presence of different organic compounds at ppt levels, which demonstrates the reliability of this extraction technique for quantitative analysis.